From Calf Intestine
Product No. P4978
Store at –20 °C
CIP is commonly used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Removal of 5’-phosphates is very useful in preventing self-ligation of cleaved DNA vectors. This property greatly reduces background (plasmids without insert) in cloning procedures.
Activity: approx. 10,000 units/mL (see label for lot specific activity)
DNase: None detected
Nickase: None detected
RNase: None detected
Unit Definition: One unit will hydrolyze 1.0 µmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C (diethanolamine).
Greater than 95% of the activity can be inactivated by heating to 75 °C for 10 minutes in the presence of 5 mM EDTA.
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