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High-Throughput 3D Cell Culture Using TrueGel3D® HTS Hydrogel Plates

The TrueGel3D® HTS Hydrogel Plate is a ready-to-use solution to easily establish 3D cell cultures using fully synthetic hydrogels in a simple and automation-compatible manner.

HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications

Hystem 3D hydrogels are based on hyaluronic acid, a major component of the extracellular matrix. 3D cell culture can be done using Hystem hydrogels.

MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications

Human cell derived basement membrane extract (BME) hydrogel for 3D cell culture of primary cell, stem cell and xenograph cancer models.

Millicell^® Microwell Plates Frequently Asked Questions (FAQs)

Explore answers to your frequency asked questions about Millicell® Microwell plates. Our plates support reliable organoid culture with array of ultra-dense microwells in hydrogel for immunology, drug development, and cell therapy applications.

Millicell^® Ultra-Low Attachment (ULA) Plate FAQs

Learn how to grow spheroid cultures in ULA plates, the common applications and benefits of ULA plates for 3D cell culture, Millicell® ULA plate dimensions, plate handling, troubleshooting, and more.

Evaluation of Spheroid Formation and Robustness using Millicell^® Ultra-low Attachment Plates

Learn how to quantify spheroid formation, roundness, and circularity. Discover Millicell® Ultra-low Attachment plates, an ideal 3D cell culture model for uniform spheroid formation.

Organoid Culture Frequently Asked Questions

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Downloadable 96 Well Plate Templates

We offer downloadable and printable 24, 48 & 96 well plate templates in PDF, JPG, PNG and SVG formats for design experiments performed in a well plate

Angiogenesis Assays

Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology.

Millicell^® ERS 3.0 Digital Voltohmmeter FAQs

Find answers to frequently asked questions about the Millicell® ERS 3.0, from probe depth adjustment to instrument maintenance and troubleshooting.

Neurite Outgrowth Assays

Optimized cell based neurite outgrowth assays and reagents to study neuron function and development.

Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods

This article shows a comparison of the quantitative viability bioassays MTT, alamarBlue™, and Guava® ViaCount®.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Autophagy Assays

Cell based autophagy assays including live cell LC3 GFP/RFP lentiviral biosensors, kits for autophagy detection and autophagy activators and inhibitors.

Tips and Tricks: Millicell^® DCI Digital Cell Imager Cloud Application

Tips and tricks for using the Millicell® Cloud Application to analyze data from the Millicell® DCI digital cell imager.

Human Renal Proximal Tubule Epithelial Cells (RPTEC) as an Essential Advanced Model to Express Kidney Drug Transporters and Toxicity

Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal

Size-based Immunomonitoring using the Scepter™ 3.0 Handheld Cell Counter

The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s

EX-CELL^® Advanced™ CHO Fed-Batch System – Bioprocessing Technology Institute, Singapore Case Study

In this study, nine commercially available, chemically defined media and feed combinations from different vendors were assessed for producing monoclonal antibody in Chinese hamster ovary (CHO) cells.

Impact of DMSO And Freezing Technique in Developing a High Cell Density Cryopreservation Process for Upstream Bioprocessing

Understand how considerations such as DMSO concentrations and freezing techniques are key in the successful implementation of high cell density cryopreservation (HCDC).

Modified Amino Acids

Modified amino acids are inhouse manufactured amino acid derivatives with specific properties enabling the intensification of cell culture processes.

Frequently Asked Questions About Gamma-Irradiated FBS (Serum) and Other Products for Cell Culture

Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.

Mycoplasma Clearance Performance of Stericup^® and Steritop^® Filters

Evaluation of mycoplasma removal by sterilizing filtration using 0.1 µm PES and PVDF filters for cell culture media and buffer preparation.

Protein Binding in Sterile Filtration of Cell Culture Media

Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.

Sterile Filter Selection for Cell Culture Media Preparation

Select the right sterile filter by media, volume, and application.

Validated Animal Component-Free Growth Factors for Cell Culture

Discover validated bioactivity and proliferation data for our portfolio of animal component-free cell culture growth factors.

β-Alanine in Cell Culture

Learn more about β-Alanine, an important amino acid supplement and explore how it is used in cell culture. We offer a comprehensive portfolio of amino acid cell culture supplements and reagents.

Poly-lysine Types for Biomedical Applications

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

Live Cell Imaging of Autophagy with LentiBrite™

HT-1080 cells transduced with LentiBrite™ GFP-LC3 lentiviral particles induce autophagy, aiding research in cellular processes.

Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.

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