Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system (Fig 1.11).
Figure 1.11.Example of the pressure in different parts of a system during run of a column. Note that the pressure values are only used to illustrate the principle.
For more information, refer to the ÄKTATM Laboratory-scale Chromatography Systems Instrument Management Handbook, 29-0108-31.
To protect the column hardware and the packed bed of the chromatographic medium, it is important to set limits that must not be exceeded during the run. There are two important pressure limits that must be taken into consideration:
Figure 1.12.Column hardware pressure limit is the maximum pressure the column can withstand without damage.
Figure 1.13.The maximum pressure over the packed bed is the maximum pressure the packed bed of chromatography medium can withstand without gap formation. This is not a fixed value.