Chemiluminescent immunoassays (CLIA) combine the advantages of chemiluminescence sensitivity with the specificity of an immunoreaction. In this technique, an enzyme converts a substrate into a chemiluminescent signal that is emitted as photons of light in the visible or near visible range. The amount of light produced correlates to the amount of target analyte present in the sample.
CLIA assays provide a sensitive, high-throughput alternative to conventional colorimetric ELISA methods. Although the underlying principles are the same, the colorimetric substrate is replaced by a chemiluminescent substrate in CLIA assays.
In general, chemiluminescent substrates should provide the following advantages:
* Microplate selection: to develop a reliable CLIA assay, it is crucial to select a microplate that is suitable for luminescence. Plates are usually opaque white with solid white or clear-bottomed wells to avoid background and cross talk.
Ultimately, the target analyte is quantified by measuring the light intensity generated by the chemiluminescent immunoreaction. Luminescence is measured in Relative Light Units (RLU) by a luminometer, where RLUs are typically proportional to the amount of target analyte present in the sample.
Importantly, CLIA assays are one of the most sensitive detection methods due to signal multiplication and amplification. The technique can detect small concentrations of biological molecules (limit of detection = zeptomole 10-21 mol). In the presence of enhancers, chemiluminescent reactions occur for even longer without a reduction in the light output. Substrate can be added only minutes before detection and there is no requirement for a long incubation period nor a need for stopping reagents, as is the case with conventional colorimetric ELISA assays.
Find detailed protocols to perform direct and indirect CLIA and ELISA assays.
Enzymes, Luminophores, Substrates and Enhancers – Enabling your chosen detection method
Antibody Portfolio and Custom Services – Take advantage of a broad product offering, supported by the expertise and customization you require
Reagents for Antibody and Protein Production – Helping you manufacture the assay components you need
Blocking Agents – Secure the quality reagents you require for your assay's performance
Estapor® Magnetic and Polymeric Microspheres – Benefit from a consistent, sensitive and flexible range of assay enhancing options. Discover the possibilities in assay automation and purification through:
Off-the-Shelf Chemiluminescent Kits – Customizable according to your needs
One-Stop Shop for Ancillary Reagents – Simplicity and convenience to accelerate your commercialization
Download our brochure for a comprehensive list of ELISA kits and to search ELISA analysis kits by target or gene name which can be customized based on your CLIA assay needs.
Accelerate your time to market with fit-for-use products which offer the quality, consistency, and documentation necessary for every step of your IVD development and manufacturing needs.
Leverage our extensive immunoassay development knowledge, based on in-depth product development and manufacturing experience, and supported by IVD industry partnerships.
By partnering with us early in development to source raw materials, you help to ensure a smooth and efficient transfer from R&D through to commercialization.
In addition to letting you design and order your custom DNA and RNA oligos and qPCR probes, we offer years of experience in critical and non-critical raw material manufacturing, supporting a full range of custom and contract manufacturing services for diagnostic manufacturers.
Let us partner with you from the development of the initial concept, through to scale up and final manufacturing. We ensure that you have quality products, at the optimal scale, at the right time, to aid your rapid commercialization.
To continue reading please sign in or create an account.Don't Have An Account?