May be used for washing spheroplasts and in isoelectric focusing to minimize endoosmotic flow in agarose gels. May be used to induce osmotic stress.
Sorbitol, a bacterial culture supplement, has been used for the preparation of selective plating media to culture Sorbitol-fermenting bacteria including strains of Escherichia coli and Yersinia enterocolitica. It has also been used as one of the ingredients to prepare Drosophila embryos for immunoelectron microscopy. It has also been used as an additive to glucuronoxylan as potential food packaging material.
Acciones bioquímicas o fisiológicas
D-Sorbitol is a sugar alcohol that is commonly used as a sugar substitute. It occurs naturally and is also produced synthetically from glucose. The food industry uses D-sorbitol as an additive in the form of a sweetener, humectant, emulsifier, thickener, or dietary supplement. D-Sorbitol has also been found in cosmetics, paper, and pharmaceuticals. Naturally, D-sorbitol occurs widely in plants via photosynthesis, ranging from algae to higher order fruits of the family Rosaceae.
Sorbitol is a slowly metabolized sugar alcohol produced by reduction of glucose. In the polyol pathway, sorbitol is further oxidized to fructose by sorbitol dehydrogenase. Sorbitol does not diffuse easily across the cell membranes and may cause osmotic damage to the cells. The metabolism of sorbitol is most important in the pathology of diabetes related vascular complications.