Merck
All Photos(3)

302643

Sigma-Aldrich

Trypan Blue

Dye content 60 %

Synonym(s):
Direct blue 14
Linear Formula:
C34H24N6O14S4Na4
CAS Number:
Molecular Weight:
960.81
Número de índice de color:
23850
Beilstein:
4360496
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.47

Nivel de calidad

200

formulario

powder

composición

Dye content, 60%

mp

>300 °C (lit.)

solubilidad

H2O: 1 mg/mL

λmax

607 nm

ε (extinction coefficient)

≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

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Related Categories

Aplicación

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Envase

25, 100 g in glass bottle

Acciones bioquímicas o fisiológicas

Trypan blue is a blue acid dye that contains two azo chromophores. It is a large, hydrophilic, tetrasulfonated anionic dye. It is also used to screen the cytotoxic properties of plant extracts. In addition, trypan blue is used for staining epiretinal membranes during vitrectomy surgery.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Carc. 1B

Código de clase de almacenamiento

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Equipo de protección personal

Eyeshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificate of Analysis

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Certificate of Origin

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