05-1242

Sigma-Aldrich

Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4

clone 6F12-H4, from mouse

Sinónimos:
H3K9me3, Histone H3 (tri methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
eCl@ss:
32160702
NACRES:
NA.41

Nivel de calidad

100

origen biológico

mouse

antibody product type

primary antibodies

clon

6F12-H4, monoclonal

species reactivity

mouse, human

aplicaciones

ChIP: suitable (ChIP-seq)
dot blot: suitable
immunofluorescence: suitable
inhibition assay: suitable (peptide)
western blot: suitable

isotipo

IgG1κ

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice

Descripción general

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely via acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α/β. The enzymes responsible for H3K9me3 formation are SUV39H1 and SUV39H2.

Especificidad

Based on sequence homology, broad species cross-reactivity is expected with all mammals, Drosophila, Xenopus, and Arabidopsis.

Aplicación

Use Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4 (mouse monoclonal antibody) validated in ChIP, PIA, IF, DB, ChIP-seq, WB to detect trimethyl-Histone H3 (Lys9) also known as H3K9me3, Histone H3 (tri methyl K9).
Chromatin Immunoprecipitation (ChIP):
Representative data from a previous lot. Sonicated 3T3 L1 chromatin was subjected to chromatin immunoprecipitation using anti- trimethyl-histone H3 (Lys9) and the Magna ChIP G (Cat. #17-611) Kit. Successful immunoprecipitation of trimethylhistone H3 (Lys9) associated DNA fragments was verified by qPCR using primers flanking the p16 promoter.

Peptide Inhibition Analysis:
Peptide blocking assay demonstrates distinct preference of the antibody for the trimethyl form vs. the dimethyl form.


Chromatin Immunoprecipitation (ChIP):
ChIP analysis of known chromosomal Suv39h targets (H3K9me3 in major satellites, mouseES cells).


Dot Blot Analysis:
Dot-blot analysis demonstrating specificity of anti-H3K9me3, clone 6F12-H4 for trimethyl Lys9 of histone H3.

Calidad

Routinely evaluated by Western Blot on HeLa acid extracts.

Western Blot Analysis: A 0.5 – 5 μg dilution of this lot detected trimethyl histone H3 (Lys9) in HeLa acid extracts.

Descripción de destino

~17 kDa

Forma física

Format: Purified
Melissa Suter et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(2), 714-726 (2010-11-26)
The effect of in utero exposure to a maternal high-fat diet on the peripheral circadian system of the fetus is unknown. Using mRNA copy number analysis, we report that the components of the peripheral circadian machinery are transcribed in the...
Jin Hou et al.
Cancer cell, 19(2), 232-243 (2011-02-15)
The full scale of human miRNome in specific cell or tissue, especially in cancers, remains to be determined. An in-depth analysis of miRNomes in human normal liver, hepatitis liver, and hepatocellular carcinoma (HCC) was carried out in this study. We...
Suv39h1 mediates AP-2?-dependent inhibition of C/EBP? expression during adipogenesis.
Zhang, ZC; Liu, Y; Li, SF; Guo, L; Zhao, Y; Qian, SW; Wen, B; Tang, QQ; Li, X
Molecular and cellular biology null
Differentially expressed genes are marked by histone 3 lysine 9 trimethylation in human cancer cells.
Wiencke, JK; Zheng, S; Morrison, Z; Yeh, RF
Oncogene null
Xu Wang et al.
The EMBO journal, 36(9), 1243-1260 (2017-03-23)
Enhancer of zeste homolog 2 (EZH2) has been characterized as a critical oncogene and a promising drug target in human malignant tumors. The current EZH2 inhibitors strongly suppress the enhanced enzymatic function of mutant EZH2 in some lymphomas. However, the...

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