05-163

Sigma-Aldrich

Anti-PLCγ-1 Antibody

Upstate®, from mouse

Sinónimos:
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1, Phosphoinositide phospholipase C, Phospholipase C-gamma-1, monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphoinositidase C, phospholipase C gamma 1,
eCl@ss:
32160702
NACRES:
NA.41

Nivel de calidad

100

origen biológico

mouse

antibody product type

primary antibodies

clon

B-2-5, monoclonal
B-20-3, monoclonal
B-6-4, monoclonal
D-7-3, monoclonal
E-9-4, monoclonal

species reactivity

rabbit, bovine, rat, human, mouse

manufacturer/tradename

Upstate®

aplicaciones

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice

Gene Information

human ... PLCG1(5335)

Descripción general

Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.

Especificidad

This antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992).

Inmunógeno

Five individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain.

Aplicación

Research Sub Category
Lipid Signaling
Anti-PLCγ-1 Antibody detects level of PLCγ-1 & has been published & validated for use in IC, IP & WB.
Research Category
Signaling
Immunoprecipitation:
4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.

Immunocytochemistry:
5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.

Calidad

Routinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.

Descripción de destino

135 kDa

Forma física

Liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as purified immunoglobulin. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
Format: Purified
Protein G Chromatography

Almacenamiento y estabilidad

Stable for 1 year at -20ºC from date of shipment.

Nota de análisis

Control
A431 cell lysate, HeLa cell lysate
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
J M Haugh et al.
The Journal of biological chemistry, 274(13), 8958-8965 (1999-03-20)
The epidermal growth factor receptor (EGFR) ligands, epidermal growth factor (EGF), and transforming growth factor-alpha (TGFalpha) elicit differential postendocytic processing of ligand and receptor molecules, which impacts long-term cell signaling outcomes. These differences arise from the higher affinity of the...
A Zapf-Colby et al.
Oncogene, 18(35), 4908-4919 (1999-09-22)
Nerve growth factor (NGF) treatment of Chinese hamster ovary fibroblast (CHO) cells exogenously expressing 2.5x105 TrkA receptors (CHO/TrkA) results in inhibition of serum and insulin-like growth factor-I (IGF-I) stimulated cell proliferation in a dose-dependent manner. Furthermore, NGF does not stimulate...
Yang Liu et al.
EBioMedicine, 51, 102607-102607 (2020-01-10)
PLCγ enzymes are key nodes in cellular signal transduction and their mutated and rare variants have been recently implicated in development of a range of diseases with unmet need including cancer, complex immune disorders, inflammation and neurodegenerative diseases. However, molecular...
L L Runft et al.
Developmental biology, 214(2), 399-411 (1999-10-20)
Elevation of intracellular Ca2+ at fertilization is essential for the initiation of development in the Xenopus egg, but the pathway between sperm-egg interaction and Ca2+ release from the egg's endoplasmic reticulum is not well understood. Here we show that injection...
Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida.
S J MacKenzie,N A Peres,M P Barquero,L F Arauz,L W Timmer
Phytopathology null

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