05-746R

Sigma-Aldrich

Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal

clone JY325, from rabbit

Sinónimos:
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

100

antibody product type

primary antibodies

clon

JY325, monoclonal

species reactivity

human

envase

antibody small pack of 25 μL

aplicaciones

inhibition assay: suitable (peptide)
western blot: suitable

isotipo

IgG

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

dry ice

Gene Information

human ... HIST1H3F(8968)

Descripción general

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Consistent with this role, Histone H3 phosphorylated at threonine 3 has been shown to be recognized by the BIR domain Survivin, a member of the inhibitor of apoptosis (IAP) family.

Especificidad

This antibody recognizes Histone H3 phosphorylated at Thr3.

Inmunógeno

Epitope: Phosphorylated Thr3
KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Thr3.

Aplicación

Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325 is a Rabbit Monoclonal Antibody for detection of phospho-Histone H3 (Thr3) also known as Histone H3.1 & has been validated in WB, PIA.
Peptide Block Analysis: This antibody peptide blocked on colcemid treated HeLa cell extracts.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

Calidad

Evaluated by Western Blot using colcemid treated HeLa acid extract and recombinant Histone H3 negative control.

Western Blot Analysis: 1:8,000 dilution of this antibody detected Histone H3 in 10 µg of colcemid treated HeLa acid extract.

Descripción de destino

~17 kDa observed

Forma física

Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide and 40% glycerol.
Format: Purified
Protein A

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Nota de análisis

Control
Colcemid treated HeLa acid extract and recombinant Histone H3 negative control.

Otras notas

Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificado de Análisis
Laurence de Nijs et al.
Human molecular genetics, 21(23), 5106-5117 (2012-08-29)
Heterozygous mutations in Myoclonin1/EFHC1 cause juvenile myoclonic epilepsy (JME), the most common form of genetic generalized epilepsies, while homozygous F229L mutation is associated with primary intractable epilepsy in infancy. Heterozygous mutations in adolescent JME patients produce subtle malformations of cortical...
Lama Tarayrah et al.
Biology open, 4(11), 1518-1527 (2015-10-23)
Signaling pathways and epigenetic mechanisms have both been shown to play essential roles in regulating stem cell activity. While the role of either mechanism in this regulation is well established in multiple stem cell lineages, how the two mechanisms interact...
Jing Xie et al.
Cell, 163(4), 920-933 (2015-11-03)
A long-standing question concerns how stem cells maintain their identity through multiple divisions. Previously, we reported that pre-existing and newly synthesized histone H3 are asymmetrically distributed during Drosophila male germline stem cell (GSC) asymmetric division. Here, we show that phosphorylation...
Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane.
Moraes, I; Yuan, ZF; Liu, S; Souza, GM; Garcia, BA; Casas-Mollano, JA
Testing null
David S Moura et al.
Cellular and molecular life sciences : CMLS, 75(14), 2591-2611 (2018-01-18)
Regulation of cell division requires the integration of signals implicated in chromatin reorganization and coordination of its sequential changes in mitosis. Vaccinia-related kinase 1 (VRK1) and Aurora B (AURKB) are two nuclear kinases involved in different steps of cell division....

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