05-824

Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal

clone 68-4, Upstate^®, from rabbit

• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• antibody product type: primary antibodies
• clon: 68-4, monoclonal
• species reactivity: human
• envase: antibody small pack of 25 μL
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NM_001437
• Nº de acceso UniProt: Q92731
• enviado en: ambient
• Gene Information: human ... ESR2(2100)

Descripción

Descripción general

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.

Produced in collaboration with Epitomics

Especificidad

Predicted to cross-react with rat and mouse based on sequence homology

Recognizes Estrogen Receptor β, long form, does not detect estrogen receptor alpha.

Inmunógeno

KLH-conjugated linear peptide corresponding to amino acids 108-127 from the N-terminal half of human estrogen receptor beta.

Aplicación

Detect Estrogen Receptor β also known as Nuclear receptor subfamily 3 group A member 2 with Anti-Estrogen Receptor β Antibody, clone 68-4 (Rabbit Monoclonal Antibody), that has been demonstrated to work in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Calidad

routinely evaluated by immunoblot on RIPA lysates from MCF7 cells

Descripción de destino

59 kDa

Forma física

Format: Purified

Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% sodium azide, pH 7.4.

Protein A purified

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
MCF7 cell extracts

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 1