Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker

Upstate®, from rabbit

H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)

origen biológico


Nivel de calidad


forma del anticuerpo

purified antibody

antibody product type

primary antibodies



species reactivity

human, mouse




immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice

Gene Information

human ... HIST1H3F(8968)

Descripción general

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.


This antibody recognizes Histone H3 when phosphorylated at Ser10.


Linear peptide corresponding to human Histone H3 at Ser10.
Epitope: Ser10


Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.
Beadlyte Assay Analysis: A representative lot recognizes Histone H3 phosphorylated on Ser10 by Luminex assay.
Research Sub Category
Research Category
Epigenetics & Nuclear Function
Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker is a Rabbit Polyclonal Antibody for detection of Histone H3 phosphorylated at serine 10.This highly published Ab, also known as Anti-H3S10p, has been validated in ICC, IP & WB.


Evaluated by Western Blot in Colcemid treated HeLa acid extract.
Western Blot Analysis: 0.5 – 1 µg/ml of this antibody detected Histone H3 on 10 µg of Colcemid treated HeLa acid extract.

Descripción de destino

17 kDa

Forma física

Format: Purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
Protein A purified

Almacenamiento y estabilidad

2-8°C for up to 6 months.

Nota de análisis

Colcemid treated HeLa acid extract

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


10 - Combustible liquids

WGK Alemania


Certificado de Análisis

Certificado de origen

On hemangioblasts in chicken.
Weng, W; Sukowati, EW; Sheng, G
Testing null
Fgf8 expression defines a morphogenetic center required for olfactory neurogenesis and nasal cavity development in the mouse.
Kawauchi, S; Shou, J; Santos, R; Hebert, JM; McConnell, SK; Mason, I; Calof, AL
Development null
Loss of Hspa9b in zebrafish recapitulates the ineffective hematopoiesis of the myelodysplastic syndrome.
Craven, SE; French, D; Ye, W; de Sauvage, F; Rosenthal, A
Blood null
Lyubomir T Vassilev et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(28), 10660-10665 (2006-07-05)
CDK1 is a nonredundant cyclin-dependent kinase (CDK) with an essential role in mitosis, but its multiple functions still are poorly understood at a molecular level. Here we identify a selective small-molecule inhibitor of CDK1 that reversibly arrests human cells at...
The Lim homeobox gene Lhx2 is required for olfactory sensory neuron identity.
Kolterud, A; Alenius, M; Carlsson, L; Bohm, S
Development null

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico