06-935

Anti-Estrogen Receptor α Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• antibody product type: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), rat (immunogen homology)
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_036821
• Nº de acceso UniProt: P06211
• enviado en: wet ice
• Gene Information: human ... ESR1(2099) ; mouse ... Esr1(13982) ; rat ... Esr1(24890)

Description

Descripción general

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.

Especificidad

This antibody recognizes the C-terminus of Estrogen Receptor alpha.

Inmunógeno

Epitope: C-terminus.

KLH-conjugated linear peptide corresponding to the C-terminus of rat Estrogen Receptor alpha.

Aplicación

Anti-Estrogen Receptor α Antibody is a Rabbit Polyclonal Antibody for detection of Estrogen Receptor α also known as Estrogen receptor-like 1, Estrogen-related receptor alpha & has been validated in EMSA, ICC, IHC, IP & WB.

Immunoprecipitation Analysis: 0.2 µg/µL from a representative lot detected Estrogen Receptor a in MCF7 cell lysate.

Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected Estrogen Receptor a in MCF7 cell lysate.

Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Estrogen Receptor a in human breast cancer and uterine cancer cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Hormones & Receptors

Calidad

Evaluated by Western Blot in MCF7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Estrogen Receptor a in MCF7 cell lysate.

Descripción de destino

~58 kDa observed. UniProtKB/Swiss-Prot P06211 (ESR1_RAT) lists a molecular weight of 67 kDa A molecular weight between 52-58 kDA due to variant isoforms may be observed in some cell lysates (Al-Bader, M.D. (2006). Reprod Biol Endocrinol. 4:13.; Geisler, J.G. et al. (2002). Diabetes. 51(7):2158-2169.). Uncharacterized bands may be seen at a molecular weight of ~10 kDa, ~35 kDa, ~41 kDa, ~105 kDa, and ~220 kDa in some cell lysates.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
MCF7 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable