07-330

Sigma-Aldrich

Anti-Partitioning-defective 3 Antibody

Upstate®, from rabbit

Sinónimos:
Atypical PKC isotype-specific-interacting protein, CTCL tumor antigen se2-5, atypical PKC isotype-specific interacting protein, par-3 (partitioning defective 3, C.elegans) homolog, par-3 partitioning defective 3 homolog (C. elegans), partitioning-defecti
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

100

antibody product type

primary antibodies

clon

polyclonal

species reactivity

mouse, canine, rat, human, monkey

manufacturer/tradename

Upstate®

aplicaciones

immunocytochemistry: suitable
western blot: suitable

isotipo

IgG

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

dry ice

Gene Information

human ... PARD3(56288), PHIP(55023)

Descripción general

PAR3 is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR3 interacts with PRCKI. PAR3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR3 interacts with F11R/JAM1. Antibodies against PAR3 are present in sera from patients with cutaneous T cell lymphomas.

Especificidad

Recognizes all 3 slice isoforms of PAR-3, MW ~180, 150, 100 kDa.

Inmunógeno

GST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).

Aplicación

Research Category
Cell Structure
Immunocytochemistry:
A previous lot of this antibody showed positive immunostaining for PAR-3 in MDCK cells fixed with 3% paraformaldehyde followed by permeabilization with 0.1% Triton X-100 has been shown by an independent laboratory.
Anti-Partitioning-defective 3 Antibody is an antibody against Partitioning-defective 3 and has been published in over 20 citations for use in IC and WB.
Research Sub Category
Cytoskeleton

Calidad

Evaluated by western blot on RIPA lysates from 3T3/A31, A431, and HeLa cells.

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected PAR-3 isoforms in RIPA lysates from 3T3/A31, A431, and HeLa cells.

Descripción de destino

~180, 150 & 100 kDa

Ligadura / enlace

Replaces: MABS174

Forma física

Purified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
Format: Purified
Protein A chromatography

Almacenamiento y estabilidad

Stable for 1 year at -20ºC from date of receipt.

Nota de análisis

Control
3T3/A31 lysate.

Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 µL of 2-mercapto-ethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Par proteins: partners in polarization.
Macara, Ian G
Current Biology, 14, R160-R162 (2004)
Roles of nectins in cell adhesion, migration and polarization.
Nakanishi, Hiroyuki and Takai, Yoshimi
Biological Chemistry, 385, 885-892 (2004)
Wdr1-mediated cell shape dynamics and cortical tension are essential for epidermal planar cell polarity.
Luxenburg, C; Heller, E; Pasolli, HA; Chai, S; Nikolova, M; Stokes, N; Fuchs, E
Nature Cell Biology null
Junctional adhesion molecules (JAMs): more molecules with dual functions?
Ebnet, Klaus, et al.
Journal of Cell Science, 117, 19-29 (2004)
Cdx2 regulates endo-lysosomal function and epithelial cell polarity.
Gao N, Kaestner KH
Genes & Development null

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