07-449

Sigma-Aldrich

Anti-trimethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

Sinónimos:
H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3k27me3
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

100

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

polyclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

aplicaciones

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
multiplexing: suitable
western blot: suitable

isotipo

IgG

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

dry ice

Gene Information

human ... HIST1H3F(8968)

Descripción general

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Especificidad

Broad species cross-reactivity expected.
Trimethylated histone H3 (Lys27). Broad species cross-reactivity expected.

Inmunógeno

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(me3K)SAP… in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.

Aplicación

Anti-trimethyl-Histone H3 (Lys27), also known as Anti-H3K27me3, is a highly published Rabbit Polyclonal Antibody. This protein A purified antibody is dot blot tested for trimethylated lysine 27 specificity and validated in WB, ICC, IP.
Immunocytochemistry:

Immunoprecipitation:

Beadlyte Histone-Peptide Specificity Assay:

Calidad

Routinely evaluated by western blot in acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.4 µg/mL of anti-trimethyl histone H3 Lys27 was used on recombinant Histone H3 and HeLa cell acid extracts.

Descripción de destino

17 kDa

Forma física

Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide before the addition of 30% glycerol.
Format: Purified

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

storage_class_code

10 - Combustible liquids

WGK Alemania

WGK 1

Certificado de Análisis

Certificado de origen

A WD40 domain cyclophilin interacts with histone H3 and functions in gene repression and organogenesis in Arabidopsis.
Li, H; He, Z; Lu, G; Lee, SC; Alonso, J; Ecker, JR; Luan, S
Plant Cell null
COOH-terminal binding protein regulates expression of the p16INK4A tumor suppressor and senescence in primary human cells.
Mroz, EA; Baird, AH; Michaud, WA; Rocco, JW
Cancer Research null
An extended transcriptional network for pluripotency of embryonic stem cells.
Kim, J; Chu, J; Shen, X; Wang, J; Orkin, SH
Cell null
Polycomb recruitment to DNA in vivo by the YY1 REPO domain.
Wilkinson, FH; Park, K; Atchison, ML
Proceedings of the National Academy of Sciences of the USA null
RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena.
Liu, Y; Taverna, SD; Muratore, TL; Shabanowitz, J; Hunt, DF; Allis, CD
Genes & Development null

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