1.50252

Purospher^® STAR RP-18 endcapped (5 µm) LiChroCART^® 250-4

suitable for HPLC

Properties

• material: stainless steel column
• Nivel de calidad: 100
• Agency: suitable for USP L1
• Línea del producto: Purospher^®
• característica: endcapped
• envase: 1 ea of
• extensión del etiquetado: 17% Carbon loading
• parámetro: 250 bar pressure; 65 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• columna L × D.I.: 25 cm × 4 mm
• matriz: fully porous particle
• grupo activo de la matriz: C18 bonding phase
• tamaño de partícula: 5 μm
• tamaño de poro: 120 Å pore size
• superficie: 330 m²/g
• operating pH range: 1.5-10.5
• técnica de separación: reversed phase
• temp. de almacenamiento: no temp limit

Description

Descripción general

Purospher^® STAR [https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/purospher-star-hplc-and-uhplc-columns] RP-18 end-capped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer outstanding stability from pH 1.5 to 10.5 over a wide temperature range and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate the best all-around retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher^® STAR RP-18 end-capped, 5 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase. Purospher^® STAR RP-18 end-capped, 5 µm LiChroCART^® 250-4 is a cartridge column that requires a manuCART^™ cartridge column holder part number 1.51486.0001 [https://www.sigmaaldrich.com/US/en/product/mm/151486]. The manuCART^™ can hold one cartridge column with or without direct integration of a 4-4 mm guard column. The recommended guard column is Purospher^®STAR RP-18 end-capped (5 µm) LiChroCART^® 4-4 part number 1.50250.0001 [https://www.sigmaaldrich.com/US/en/product/mm/150250]]. LiChroCART^® cartridge columns mounted with a manuCART^™ cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher^® STAR RP-18 endcapped, 5 µm Hibar^® RT 250-4 HPLC columns are available part number 1.150037.0001 [https://www.sigmaaldrich.com/US/en/product/mm/150037m].The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for high-resolution HPLC separation of complex samples.Item 1.50252.1003 is a Validation kit containing 3 Purospher^® STAR RP-18 endcapped, 5 µm LiChroCART^® 250-4 cartridge columns from 3 different base material lots.Learn more about Purospher^® STAR HPLC and UHPLC Columns [https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/408/631/puropsher-star-hplc-uhplc-brochure-br4748en-ms.pdf]Learn more about the Supelco^® HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide [https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/600/187/hplc-columns-guide-br7614en-ms.pdf]Brochure: A Practical Guide to HPLC - Method Development Guide [https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/446/037/hplc-method-dev-guide-br6818en-ms.pdf]

Aplicación

Intended for the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat-soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water-soluble vitamins.

Características y beneficios

• Good suitability for LC-MS applications

Nota de análisis

Pressure: ≤ 130 bar
Theoretical Plates (N/m) (Toluene): ≥ 80000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.5

Información legal

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany

MANUCART is a trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Supelco is a registered trademark of Sigma-Aldrich Co. LLC