Merck
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1.50653

Supelco

Purospher® STAR RP-18 endcapped (3µm) Hibar® HR 100-2.1

suitable for UHPLC

material

stainless steel column

Nivel de calidad

Agency

suitable for USP L1

Línea del producto

Purospher®

característica

endcapped

envase

1 ea of

extensión del etiquetado

17% Carbon loading

parámetro

1000 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

columna L × D.I.

10 cm × 2.1 mm

superficie

330 m2/g

matriz

fully porous particle

grupo activo de la matriz

C18 bonding phase

tamaño de partícula

3 μm

tamaño de poro

120 Å pore size

operating pH range

1.5-10.5

técnica de separación

reversed phase
supercritical fluid (SFC)

temp. de almacenamiento

no temp limit

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Este artículo
1.506541.503981.50607
agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

product line

Purospher®

product line

Purospher®

product line

Purospher®

product line

Purospher®

feature

endcapped

feature

endcapped

feature

endcapped

feature

endcapped

packaging

1 ea of

packaging

1 ea of

packaging

1 ea of

packaging

1 ea of

extent of labeling

17% Carbon loading

extent of labeling

17% Carbon loading

extent of labeling

17% Carbon loading

extent of labeling

17% Carbon loading

Descripción general

Purospher® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B).
These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test.

Purospher® STAR RP-18 endcapped, 3 µm Hibar® HR 100-2.1 is a ready to use UHPLC column suitable for connection to any UHPLC system providing a pressure stability of 1000 bar.
The column dimension of 100 mm length x 2.1 mm I.D. is the optimal choice for fast and high resolution UHPLC separation of complex samples allowing significant solvent savings and improved sensitivity. A 2.1 mm I.D. column enables a more sustainable separations in comparison to 4, 4.6 or 3 mm I.D. column due to the use of significant less solvent for the chromatographic separation.

Learn more about Purospher® STAR HPLC and UHPLC Columns
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Aplicación

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Características y beneficios

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Nota de análisis

Pressure: ≤ 120 bar
Theoretical Plates (N/m) (1,2-Benzanthracene): ≥ 130000
Symmetry (1,2-Benzanthracene): 0.8 - 1.4
Capacity factor (1,2-Benzanthracene): 7.0 - 10.0

Información legal

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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Artículos

HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped

Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators, notably in the treatment of asthma symptoms. This application shows the efficient separation of several common xanthines and may be applied their analysis in any number of desired matrices.

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