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219466

Millipore

CELLULYSIN® Cellulase, Trichoderma viride

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Sinónimos:
β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase
Número de CAS:
9012-54-8
Comisión internacional de enzimas:
3.2.1.4 (BRENDA, IUBMB)
Número MDL:
MFCD00081510

formulario

solid

specific activity

≥10,000 units/g dry wt.

manufacturer/tradename

Calbiochem®

condiciones de almacenamiento

OK to freeze

color

yellow-white

solubilidad

water: soluble

enviado en

ambient

temp. de almacenamiento

2-8°C

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Este artículo
C1184C854622178
Millipore

Millipore

219466

CELLULYSIN® Cellulase, Trichoderma viride

Cellulase from Aspergillus niger powder, ≥0.3 units/mg solid

Sigma-Aldrich

C1184

Cellulase from Aspergillus niger

Cellulase from Trichoderma reesei ATCC 26921 lyophilized powder, ≥1 unit/mg solid

Sigma-Aldrich

C8546

Cellulase from Trichoderma reesei ATCC 26921

Sigma-Aldrich

Sigma-Aldrich

22178

Cellulase from Aspergillus niger

specific activity

≥10,000 units/g dry wt.

specific activity

≥0.3 units/mg solid

specific activity

≥1 unit/mg solid

specific activity

~0.8 U/mg

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

color

yellow-white

color

-

color

-

color

off-white

solubility

water: soluble

solubility

-

solubility

deionized water: soluble 5.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).)

solubility

-

Descripción general

Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.

Aplicación

CELLULYSIN® Cellulase, Trichoderma viride has been used:
  • to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
  • in the enzyme mix used for in situ hybridization to plant chromosomes
  • in the enzyme solution for the transformation of Phytophthora species
  • to isolate intact cells and protoplasts from plant tissues

Acciones bioquímicas o fisiológicas

Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.

Advertencia

Toxicity: Harmful (C)

Definición de unidad

One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.

Otras notas

Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany

pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificates of Analysis (COA)

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Ejemplo

T1503
Referencia del producto
-
25G
Tamaño del envase/cantidad

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P2401

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Hemicellulase from Aspergillus niger powder, 0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate)

Sigma-Aldrich

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Sigma-Aldrich

D8037

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Farjana Islam et al.
BMC research notes, 11(1), 445-445 (2018-07-06)
This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste (molasses) and characterization by morphological and biochemical analysis. Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC)
Weizhen Wang et al.
Frontiers in microbiology, 10, 2402-2402 (2019-11-12)
Phytophthora, a genus of oomycetes, contains many devastating plant pathogens, which cause substantial economic losses worldwide. Recently, CRISPR/Cas9-based genome editing tool was introduced into Phytophthora to delineate the functionality of individual genes. The available selection markers for Phytophthora transformation, however
Man-Wah Li et al.
PloS one, 10(8), e0135033-e0135033 (2015-08-14)
Arabidopsis High Mobility Group Box (HMBG) proteins were previously found associated with the interphase chromatin but not the metaphase chromosome. However, these studies are usually based on immunolocalization analysis involving paraformaldehyde fixation. Paraformaldehyde fixation has been widely adapted to preserved
In Situ Hybridization to Plant Chromosomes
Fluorescence In Situ Hybridization (FISH), 447-494 null
Rahman Ebrahimzadegan et al.
Scientific reports, 9(1), 19989-19989 (2019-12-29)
Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (-B) and B chromosome containing

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