344905

Sigma-Aldrich

FURA 2/AM - CAS 108964-32-5 - Calbiochem

Cell-permeable ester form of FURA 2.

Sinónimos:
1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2ʹ-amino-5ʹ-methylphenoxy)-ethane-N,N,NʹNʹ-tetraacetic Acid Pentaacetoxymethyl Ester, FURA 2/AM - CAS 108964-32-5 - Calbiochem
Empirical Formula (Hill Notation):
C44H47N3O24
Número de CAS:
Peso molecular:
1001.85
Número MDL:

storage conditions

-20C

Nivel de calidad

100

ensayo

≥95% (HPLC)

formulario

solid

manufacturer/tradename

Calbiochem®

condiciones de almacenamiento

OK to freeze
protect from light

color

yellow

fluorescencia

λex 335 nm (high)
λex 362  nm (low)
λem 505 nm (high)
λem 512 nm (low)

enviado en

ambient

temp. de almacenamiento

−20°C

InChI

1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3

InChI key

UTBYGIDPBQOHNT-UHFFFAOYSA-N

Descripción general

Cell-permeable ester form of FURA 2. Note: 1 set = 10 x 100 µg.
Cell permeant ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (Kd = 224 nM) that offers 30 times greater fluorescence intensity than QUIN 2 (Cat. No. 551826). Can be used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm). Note: 1 set = 10 x 100 µg.
PLURONIC® F-127, PROTEIN GRADE Detergent (Cat. No. 540025, available separately) is a mild, low-toxicity detergent that can aid in the solubilization of FURA 2/AM.

Envase

1 set in Glass bottle
1 mg in Plastic ampoule
Packaged under inert gas

Acciones bioquímicas o fisiológicas

Primary Target
Ratiometric calcium indicator
Product does not compete with ATP.
Cell permeable: yes
Reversible: no

Advertencia

Toxicity: Standard Handling (A)

Nota de preparación

Further dilute with aqueous buffers just prior to use.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 1 month at -20°C.

Otras notas

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Pluronic is a registered trademark of BASF

storage_class_code

11 - Combustible Solids

WGK Alemania

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

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