AB15348

Sigma-Aldrich

Anti-FMRFamide Antibody

serum, Chemicon®

eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

100

forma del anticuerpo

serum

antibody product type

primary antibodies

clon

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

aplicaciones

immunohistochemistry: suitable

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

dry ice

Inmunógeno

FMRFamide

Aplicación

Research Category
Neuroscience
Research Sub Category
Hormones & Receptors
Immunohistochemistry: 1:1,000 - 1:5,000

Application Notes for Immunohistochemistry:
- Suggested perfusion steps:
1) Ca2+-free Tyrode′s solution
2) Paraformaldehyde-picric acid fixative
3) 10% sucrose in PBS as a cryo-protectant
- Rapidly dissect tissue and store overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
- Incubate slide-mounted tissue sections with blocking buffer for 1 hour at room temperature.
- Remove blocking buffer and incubate the slides with the primary antibody (AB15348 diluted to desired working concentration in blocking buffer) for 18-24 hours at 2-8°C.
- Remove primary antibody and rinse 3 times in PBS.
- Incubate sections with fluorescently labeled secondary antibody for 60 minutes at room temperature.

Optimal working dilutions must be determined by the end user.
Anti-FMRFamide Antibody is an antibody against FMRFamide for use in IH.

Forma física

Liquid with 0.05% sodium azide.
Serum

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania

WGK 1

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Tigran P Norekian et al.
The Journal of comparative neurology, 528(7), 1231-1254 (2019-11-22)
Cnidaria is the sister taxon to bilaterian animals, and therefore, represents a key reference lineage to understand early origins and evolution of the neural systems. The hydromedusa Aglantha digitale is arguably the best electrophysiologically studied jellyfish because of its system...
Ryan E Hulett et al.
Proceedings. Biological sciences, 287(1931), 20201198-20201198 (2020-07-23)
The origin of bilateral symmetry, a major transition in animal evolution, coincided with the evolution of organized nervous systems that show regionalization along major body axes. Studies of Xenacoelomorpha, the likely outgroup lineage to all other animals with bilateral symmetry...
null
Nagayasu Nakanishi et al.
Development (Cambridge, England), 139(2), 347-357 (2011-12-14)
As a sister group to Bilateria, Cnidaria is important for understanding early nervous system evolution. Here we examine neural development in the anthozoan cnidarian Nematostella vectensis in order to better understand whether similar developmental mechanisms are utilized to establish the...

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