Anti-DFF45/35 Antibody, NT

Chemicon®, from rabbit


origen biológico


Nivel de calidad


antibody product type

primary antibodies



species reactivity





immunoprecipitation (IP): suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice


Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (Liu et al. 1997). Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD (Enari et al. 1998; Sakahira et al. 1998). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (Enari et al. 1998; Sakahira et al. 1998). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which cause DNase activation and DNA degradation, is the hallmark of apoptotic cell death (Wylile et al. 1998).


Polyclonal antibody raised against a peptide corresponding to amino acids 2-21 at N-terminus of human DFF (Liu et al. 1997).
Epitope: N-terminus


This Anti-DFF45/35 Antibody, N-terminus is validated for use in IP, WB for the detection of DFF45/35.
Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Western blot: 1:500-1:1000


Whole cell lysate from HeLa cells can be used as a positive control and a 45 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Forma física

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Format: Purified

Almacenamiento y estabilidad

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania


Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Apoptosis. An endonuclease at last.
A Wyllie
Nature, 391(6662), 20-21 (1998-01-09)
M Enari et al.
Nature, 375(6526), 78-81 (1995-05-04)
Fas is a type-I membrane protein that transduces an apoptotic signal. Binding of Fas ligand or agonistic anti-Fas antibody to Fas kills the cells by apoptosis. Studies in the nematode Caenorhabditis elegans have suggested that proteases such as interleukin-1 beta-converting...
H Sakahira et al.
Nature, 391(6662), 96-99 (1998-01-09)
Various molecules such as cytokines and anticancer drugs, as well as factor deprivation, rapidly induce apoptosis (programmed cell death), which is morphologically characterized by cell shrinkage and the blebbing of plasma membranes and by nuclear condensation. Caspases, particularly caspase 3...

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