AB16965

Sigma-Aldrich

Anti-ICAD/DFF Antibody, NT (a.a. 2-21 of mouse ICAD)

Chemicon®, from rabbit

Sinónimos:
Inhibitor of Caspase Activated Deoxyribonuclease, DFF
eCl@ss:
32160702

origen biológico

rabbit

Nivel de calidad

100

antibody product type

primary antibodies

clon

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

aplicaciones

western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice

Especificidad

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated Dnase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (Liu et al. 1997). Mouse homologue of human DFF was identified as a Dnase inhibitor designated ICAD (Enari et al. 1998; Sakahira et al. 1998). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (Enari et al. 1998; Sakahira et al. 1998). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which cause Dnase activation and DNA degradation, is the hallmark of apoptotic cell death (Wylile et al. 1998).

Inmunógeno

Epitope: N-terminus (a.a. 2-21 of mouse ICAD)
Polyclonal antibody raised against a peptide corresponding to amino acids 2-21 of mouse ICAD (Enari et al. 1998).

Aplicación

Anti-ICAD/DFF Antibody, N-terminus (a.a. 2-21 of mouse ICAD) is an antibody against ICAD/DFF for use in WB.
Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Western blot: 1:500-1:1000

Mouse lung tissue lysate can be used as a positive control and a 45 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Forma física

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Format: Purified

Almacenamiento y estabilidad

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis
Apoptosis. An endonuclease at last.
A Wyllie
Nature, 391(6662), 20-21 (1998-01-09)
H Sakahira et al.
Nature, 391(6662), 96-99 (1998-01-09)
Various molecules such as cytokines and anticancer drugs, as well as factor deprivation, rapidly induce apoptosis (programmed cell death), which is morphologically characterized by cell shrinkage and the blebbing of plasma membranes and by nuclear condensation. Caspases, particularly caspase 3...
M Enari et al.
Nature, 375(6526), 78-81 (1995-05-04)
Fas is a type-I membrane protein that transduces an apoptotic signal. Binding of Fas ligand or agonistic anti-Fas antibody to Fas kills the cells by apoptosis. Studies in the nematode Caenorhabditis elegans have suggested that proteases such as interleukin-1 beta-converting...

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico

Redes sociales

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Investigación. Desarrollo. Producción.

Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos

© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.

Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.