ABC821

Sigma-Aldrich

Anti-AMBRA1 Antibody

from rabbit, purified by affinity chromatography

Sinónimos:
Activating molecule in BECN1-regulated autophagy protein 1, AMBRA1
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

100

antibody product type

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

species reactivity

rat, human

aplicaciones

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

Nº de acceso UniProt

enviado en

wet ice

Gene Information

human ... AMBRA1(55626)

Descripción general

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation (3), is in turn regulated by AMBRA1. AMBRA1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of AMBRA1 in cultured cells though RNA interference decreased the level of rapamycin and nutrient starvation-induced autophagy (4). Multiple isoforms of AMBRA1 are known to exist.

Inmunógeno

KLH-conjugated synthetic peptide corresponding to the N-terminus of human AMBRA1.
Epitope: N-terminus

Aplicación

Immunohistochemistry Analysis: 5 μg/mL from a representative lot detected AMBRA1 in human brain tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected AMBRA1 in human brain cells.
Anti-AMBRA1 Antibody is an antibody against AMBRA1 for use in western blotting, IHC & immunofluorescence.
Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer

Calidad

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 μg/mL of this antibody detected AMBRA1 in rat brain tissue lysate.

Descripción de destino

~150 kDa observed. Uncharacterized bands may be observed in some lysate(s). Uniprot describes 6 isoforms produced by alternative splicing ranging between ~84 kDa and ~143 kDa

Forma física

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Daniela Strobbe et al.
British journal of pharmacology, 178(2), 298-311 (2020-10-11)
The mitochondrial F1 Fo -ATPsynthase is pivotal for cellular homeostasis. When respiration is perturbed, its mode of action everts becoming an F1 Fo -ATPase and therefore consuming rather producing ATP. Such a reversion is an obvious target for pharmacological intervention...

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico

Redes sociales

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Investigación. Desarrollo. Producción.

Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos

© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.

Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.