ABE1952

Sigma-Aldrich

Anti-CRTC1

from rabbit

Sinónimos:
CREB-regulated transcription coactivator 1, Mucoepidermoid carcinoma translocated protein 1, Transducer of regulated cAMP response element-binding protein 1, TORC-1, Transducer of CREB protein 1
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

100

antibody product type

primary antibodies

clon

polyclonal

species reactivity

mouse, rat

species reactivity (predicted by homology)

human (based on 100% sequence homology)

aplicaciones

immunoprecipitation (IP): suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

ambient

Descripción general

CREB-regulated transcription coactivator 1 (UniProt: Q6UUV9; also known as Mucoepidermoid carcinoma translocated protein 1;Transducer of regulated cAMP response element-binding protein 1;TORC-1;Transducer of CREB protein 1) is encoded by the CRTC1 (also known as MECT1; TORC1; WAMTP1; KIAA0616) gene (Gene ID 23373) in human. CRTC1 is a 67.3 kDa protein that activates transcription through binding cAMP response element (CRE) sites on DNA. It is highly expressed in adult and fetal brain and is localized to specific regions such as the prefrontal cortex and cerebellum. Very low expression is observed in heart, spleen, lung, skeletal muscle, salivary gland, ovary, and kidney. Phosphorylation/dephosphorylation states of Ser151 are required for regulating transduction of CREB activity. It is mainly localized in the cytoplasm when phosphorylated by salt-induced kinase (SIK) or AMPK and when sequestered by 14-3-3 proteins. It is translocated to the nucleus upon dephosphorylation on Ser151. CRTC1 is shown to mediate early beta-amyloid-induced transcriptional and memory deficits and overexpression of CRTC1 is shown to rescue amyloid-induced transcriptional and cognitive deficits. CRTC1 is reported to ameliorate hippocampal-dependent learning and memory deficits in APPSw,Ind mice by enhancing the expression of a specific subset of CCTC1 target genes. A chromosomal aberration involving CRTC1 has been reported in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas.

Especificidad

ABE1952 detected a target band in lysates from mouse and rat CRTC1-transfected HEK293 cells.

Inmunógeno

GST-tagged recombinat 135-amino acid fragment from the N-terminal region of human CRTC1.

Aplicación

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected CRTC1 in 10 µg of rat hippocampus lysate.

Immunoprecipitation Analysis: 4 µL from a representative lot immunoprecipitated exogenously expressed mouse CRTC1 from transfected HEK293 cells (Courtesy of Dr Carlos A. Saura, Institut de Neurociències, Universitat Autònoma de Barcelona, Spain).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected exogenously expressed mouse and rat CRTC1 in transfected HEK293 cells, as well as endogenous CRTC1 in mouse and rat hippocampus tissue homogenates (Courtesy of Dr Carlos A. Saura, Institut de Neurociències, Universitat Autònoma de Barcelona, Spain).
Anti-CRTC1 Antibody, Cat. No. ABE1952, is a highly specific rabbit polyclonal antibody that has been validated for use in Immunoprecipitation and Western Blotting.

Calidad

Evaluated by Western Blotting in mouse hippocampus tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CRTC1 in 10 µg of mouse hippocampus tissue lysate.

Descripción de destino

~78 kDa observed. 67.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Otras notas

Concentration: Please refer to lot specific datasheet.

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

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