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ABE1971-I

Sigma-Aldrich

Anti-phospho TFEB (Ser142)

from rabbit, purified by affinity chromatography

Sinónimos:

Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

human

reactividad de especies (predicha por homología)

bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

envase

antibody small pack of 25 μL

técnicas

western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

modificación del objetivo postraduccional

phosphorylation (pSer142)

Información sobre el gen

human ... TFEB(7942)

Descripción general

Transcription factor EB (UniProt: P19484; also known as Class E basic helix-loop-helix protein 35, bHLHe35) is encoded by the TFEB (also known as BHLHE35) gene (Gene ID: 7942) in human. TFEB is a basic Helix-Loop-Helix (bHLH) leucine zipper transcription factor that plays a major role in the regulation of lysosomal biogenesis. TFEB is also a main player in the transcriptional response to starvation and controls autophagy by positively regulating autophagosome formation and autophagosome-lysosome fusion. TFEB is phosphorylated by mTORC1 and ERK at Ser142, which prevents its nuclear translocation. Pharmacological inhibition of mTORC1, as well as starvation and lysosomal disruption, activates TFEB nuclear translocation. Similarly, the serine-threonine kinase RIP1/RIPK1 is shown to suppress basal autophagic flux by activating ERK, which in turn phosphorylates TFEB at Ser142 and thereby prevents TFEB-mediated transcription of gene products involved in autophagy.

Especificidad

This rabbit polyclonal antibody specifically detects Transcription factor EB phosphorylated on Serine 142 in human cells.

Inmunógeno

KLH-conjugated linear peptide corresponding to 15 amino acids surrounding phosphoserine 142 from the N-terminal half of human Transcription factor EB, isoform 1.

Aplicación

Anti-phospho TFEB (Ser142), Cat. No. ABE1971-I, is a highly specific rabbit polyclonal antibody that targets Transcription factor EB phosphorylated on Serine 142 and has been tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function

Calidad

Evaluated by Western Blotting in transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho TFEB (Ser142) in lysate of HEK293 cells transfected with FLAG-tagged wild type TFEB.

Descripción de destino

~65 kDa observed; 52.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Clase de riesgo para el agua (WGK)

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Yuzuki Kano et al.
Kidney360, 5(2), 182-194 (2023-12-08)
Collectrin serves as a chaperone for the trafficking of neutral amino acid (AA) transporters in the apical membranes of proximal tubular cells (PTCs). Cltrn knockout reduced AAs influx into PTCs, inactivated mTOR, activated transcription factor EB, improved lysosome function, and
Zhi-Hang Li et al.
Journal of cellular and molecular medicine, 25(12), 5729-5743 (2021-05-06)
Cyclosporine A (CsA) is an immunosuppressor widely used for the prevention of acute rejection during solid organ transplantation. However, severe nephrotoxicity has substantially limited its long-term usage. Recently, an impaired autophagy pathway was suggested to be involved in the pathogenesis
Jin-Feng Zhao et al.
iScience, 27(8), 110432-110432 (2024-07-31)
Reversible phosphorylation of the transcription factor EB (TFEB) coordinates cellular responses to metabolic and other stresses. During nutrient replete and stressor-free conditions, phosphorylated TFEB is primarily localized to the cytoplasm. Stressor-mediated reduction of TFEB phosphorylation promotes its nuclear translocation and
Lizhen Cheng et al.
Cell communication and signaling : CCS, 21(1), 91-91 (2023-05-05)
Diabetic encephalopathy (DE) is a complication of type 2 diabetes mellitus (T2DM) that features Alzheimer's disease (AD)-like pathology, which can be degraded by the autophagy-lysosome pathway (ALP). Since transcription factor EB (TFEB) is a master regulator of ALP, TFEB-mediated ALP
Leon Tejwani et al.
The Journal of clinical investigation, 133(16) (2023-06-29)
Protein aggregation is a hallmark of many neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Although mutations in TARDBP, encoding transactive response DNA-binding protein 43 kDa (TDP-43), account for less than 1% of all ALS cases, TDP-43-positive aggregates are present in

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