ABN37

Sigma-Aldrich

Anti-Homer1 Antibody

from rabbit, purified by affinity chromatography

Sinónimos:
Homer protein homolog 1, Homer-1, Homer1
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

100

antibody product type

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

monkey (based on 100% sequence homology), horse (based on 100% sequence homology)

aplicaciones

immunohistochemistry: suitable (paraffin)
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice

Gene Information

human ... HOMER1(9456)

Descripción general

Homer protein homolog 1 (UniProt Q86YM7; also known as Homer-1, Homer1) is encoded by the HOMER1 (also known as SYN47) gene (Gene ID 9456) in human. Homer-1, -2, and -3 constitute a family of postsynaptic scaffold proteins that play a central role in calcium signaling. Homer members share a conserved N-terminal Ena/VASP homology 1 (EVH1) domain (a.a. 1-110 of Homer-1) that mediate signaling complexes assembly by interacting with PPXXFR proline-rich consensus sequences found in various other scaffolding and signal transduction molecules, including metabotropic glutamate receptors (mGluRs) and 1,4,5-trisphosphate receptors (IP3Rs). In addition to mediate group I mGluRs signaling via their EVH1 domain, Homer-1 spliced variants Vesl-1L, 1b, and 1d, but not Vesl-1M or Vesl-1S, also contain a C-terminal coiled-coil (a.a. 181-352) region that mediate self-multimerization. Homer-1 variants are associated with the etiology of many neurological diseases, such as chronic pain, mental retardation syndromes, Alzheimer′s disease (AD), Parkinson′s disease (PD), schizophrenia, drug-induced addiction, and traumatic brain injury (TBI). Homer1b/c-knockdown is reported to protect against chemically induced seizure in mice as a result of preventing mGluR5-mediated mTOR pathway activation.

Especificidad

Broad species reactivity expected based on high sequence homology.
This rabbit polyclonal antibody targets an internal sequence within the N-terminal half of human Homer1 isoform 1b and mouse isoform Vesl-1L. Immunogen sequence appears near the C-terminus of human isoform 1h and mouse isoforms 1b, 1d, Vesl-1M, and Vesl-1S, but absent in human isoform 1e. Highly homologous sequence (~83%) is also found in the N-terminal half of rat isoform 1c (Vesl-1L) and near the C-terminus of rat isoforms 1a (Vesl) and 1b.

Inmunógeno

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human Homer1 isoform 1b and near the C-terminus of isoform 1h.

Aplicación

Immunohsitochemistry Analysis: A 1:500 dilution from a representative lot detected Homer1 in human and mouse cerebral cortex tissue sections.

Immunohsitochemistry Analysis: A representative lot detected significantly increased stratum radiatum and stratum oriens Homer1 immunoreactivity in floating brain sections from mice received AP-12 intraperitoneal injection (Jansone, B., et al. (2015). PLoS One. 10(6):e0127686).
Anti-Homer1, Cat. No. ABN37, is a highly specific rabbit polyclonal antibody that targets Homer protein homolog 1 and has been tested in Immunohistochemistry and Western Blotting.

Calidad

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Homer1 in 10 µg of mouse brain tissue lysate.

Descripción de destino

~47 kDa observed. 40.28/19.86 kDa (human isoform 1b/1h), 40.22/42.08/41.41/22.78/20.91 kDa (mouse isoform 1b/1d/Vesl-1L/Vesl-1M/Vesl-1S) calculated. Uncharacterized bands may be observed in some lysate(s).

Otras notas

Concentration: Please refer to lot specific datasheet.

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

A Novel 1,4-Dihydropyridine Derivative Improves Spatial Learning and Memory and Modifies Brain Protein Expression in Wild Type and Transgenic APPSweDI Mice.
Jansone, B; Kadish, I; van Groen, T; Beitnere, U; Moore, DR; Plotniece, A; Pajuste, K; Klusa, V
Testing null
Myung-Su Kang et al.
The Journal of experimental medicine, 214(10), 2947-2966 (2017-09-14)
Vaccinia-related kinases (VRKs) are multifaceted serine/threonine kinases that play essential roles in various aspects of cell signaling, cell cycle progression, apoptosis, and neuronal development and differentiation. However, the neuronal function of VRK3 is still unknown despite its etiological potential in...
Baiba Jansone et al.
Pharmacological research, 113(Pt B), 781-787 (2016-06-28)
The prevalence of Alzheimer's disease (AD) is higher in females than in males, and causes more severe cognitive, memory and behavioral impairments. Previously, in male transgenic (Tg) APPSweDI mice, we reported that the novel lipophilic 1,4-dihydropyridine (DHP) derivative AP-12 crossed...
Roger Shen Zhang et al.
eLife, 9 (2020-03-24)
Latrophilin-2 (Lphn2) and latrophilin-3 (Lphn3) are adhesion GPCRs that serve as postsynaptic recognition molecules in CA1 pyramidal neurons of the hippocampus, where they are localized to distinct dendritic domains and are essential for different sets of excitatory synapses. Here, we...
Xiao-Feng Zhao et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(40), 7593-7608 (2020-09-02)
Excessive activation of mammalian target of rapamycin (mTOR) signaling is epileptogenic in genetic epilepsy. However, the exact role of microglial mTOR in acquired epilepsy remains to be clarified. In the present study, we found that mTOR is strongly activated in...

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