AP180P

Sigma-Aldrich

Donkey Anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed

Chemicon®, from donkey

eCl@ss:
32160702
NACRES:
NA.46

origen biológico

donkey

Nivel de calidad

100

antibody product type

secondary antibodies

clon

polyclonal

species reactivity

goat

manufacturer/tradename

Chemicon®

aplicaciones

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotipo

IgG

conjugado

peroxidase conjugate

enviado en

wet ice

Categorías relacionadas

Descripción general

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Aplicación

Immunohistochemistry:
1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.
Donkey anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Goat IgG for use in ELISA, IH & WB.

Calidad

Routinely tested on Enzyme-linked Immunosorbent Assay.

ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.

Forma física

Purified donkey secondary antibody IgG in lyophilized buffer 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitution:
Reconstitute with 500 μL of sterile distilled water.

Almacenamiento y estabilidad

Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Alemania

WGK 3

Localization of sulfonylurea receptor subunits, SUR2A and SUR2B, in rat heart.
Zhou, M; He, HJ; Suzuki, R; Liu, KX; Tanaka, O; Sekiguchi, M; Itoh, H; Kawahara, K; Abe, H
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Maria Cernea et al.
Journal of molecular endocrinology, 56(1), 39-46 (2015-10-23)
Adipose tissue expansion, resulting from adipocyte hyperplasia and/or hypertrophy, is a hallmark of obesity. Adipocytes are derived from mesenchymal stem cells (MSCs) through adipogenesis, a process involving three key steps: proliferation, commitment and differentiation. Although studies have elaborated on the...
Protein expression and genetic structure of the coral Porites lobata in an environmentally extreme Samoan back reef: does host genotype limit phenotypic plasticity?
Barshis DJ, Stillman JH, Gates RD, Toonen RJ, Smith LW, Birkeland C
Molecular Ecology null
Expression and functional analyses of circadian genes in mouse oocytes and preimplantation embryos: Cry1 is involved in the meiotic process independently of circadian clock regulation.
Amano, T; Matsushita, A; Hatanaka, Y; Watanabe, T; Oishi, K; Ishida, N; Anzai, M; Mitani et al.
Biology of Reproduction null
Survival and death-promoting events after transient spinal cord ischemia in rabbits: induction of Akt and caspase3 in motor neurons.
Masahiro Sakurai, Tatsuya Nagata, Koji Abe, Takashi Horinouchi, Yasuto Itoyama, Koichi Tabayashi
The Journal of Thoracic and Cardiovascular Surgery null

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico

Redes sociales

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Investigación. Desarrollo. Producción.

Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos

© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.

Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.