MAB319

Sigma-Aldrich

Anti-Chromogranin A Antibody, clone PHE5

clone PHE5, Chemicon®, from mouse

Sinónimos:
CgA, Pituitary Secretory Protein I
eCl@ss:
32160702
NACRES:
NA.41

Nivel de calidad

100

origen biológico

mouse

clon

PHE5, monoclonal

species reactivity

monkey, human

should not react with

rat, mouse

manufacturer/tradename

Chemicon®

aplicaciones

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotipo

IgG1

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice

Gene Information

human ... CHGA(1113)

Especificidad

Chromogranin A. The antibody has been extensively documented on immunohistochemical studies in deparaffinized methacarn fixed tissue, where it is a sensitive marker of neuroendocrine cells and tumors. Not recommended for formalin fixed tissues, see MAB5268 or CBL219. On western blots, it identifies the identical bands recognized by antibody LK2H10 (Wilson and Lloyd).

Inmunógeno

Human pheochromocytoma

Aplicación

Anti-Chromogranin A Antibody, clone PHE5 detects level of Chromogranin A & has been published & validated for use in WB, IH(P).
Research Category
Neuroscience
Immunohistochemistry: 1:500-1:2,000 on alcohol fixed deparaffinized tissue sections (ABC peroxidase) or 1:10,000 on Methacarn-fixed deparaffinized tissue sections. Will show fine granular cytoplasmic staining associated with secretory granules.

Western Blot: recognizes a band migrating at approximately 68-75 kDa corresponding to chromogranin A.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Hormones & Receptors

Forma física

Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
Format: Purified

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Nota de análisis

Control
POSOTIVE CONTROL: Normal adrenal gland, bowel, or thyroid tissue.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Victoria Maneu et al.
Visual neuroscience, 27(5-6), 139-147 (2010-10-12)
Some evidence suggests that retinal pigment epithelium (RPE) can express nicotinic acetylcholine receptors (nAChRs) as described for other epithelial cells, where nAChRs have been involved in processes such as cell development, cell death, cell migration, and angiogenesis. This study is...
L-Type calcium channels in enterochromaffin cells from guinea pig and human duodenal crypts: an in situ study.
Lomax, et al.
Gastroenterology, 117, 1363-1369 (2019)
null
Johanna Dalmo et al.
EJNMMI research, 7(1), 6-6 (2017-01-18)
177Lu-[DOTA0, Tyr3]-octreotate (177Lu-octreotate) is used for treatment of patients with somatostatin receptor (SSTR) expressing neuroendocrine tumors. However, complete tumor remission is rarely seen, and optimization of treatment protocols is needed. In vitro studies have shown that irradiation can up-regulate the...
Gastrointestinal stromal tumors regularly express synaptic vesicle proteins: evidence of a neuroendocrine phenotype.
Bumming, P; Nilsson, O; Ahlman, H; Welbencer, A; Andersson, MK; Sjolund, K; Nilsson, B
Endocrine-Related Cancer null

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