MAB3402

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

clone GA5, Chemicon®, from mouse

Sinónimos:
GFAP
eCl@ss:
32160702
NACRES:
NA.41

Nivel de calidad

100

origen biológico

mouse

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

GA5, monoclonal

species reactivity

chicken, rat, rabbit, mouse, pig, bovine, human

manufacturer/tradename

Chemicon®

aplicaciones

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotipo

IgG1

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice

Gene Information

human ... GFAP(2670)

Descripción general

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Especificidad

Expected to cross-react with porcine, chicken, bovine, and rabbit.
The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).

Inmunógeno

Purified glial filament (Debus, E., 1983).

Aplicación

Research Sub Category
Neuronal & Glial Markers
Immunocytochemistry:
5 μg/mL of a previous lot was used.

Immunohistochemistry:
5 µg/mL

Immunoblotting:
Recognizes a 51kDa protein in reducing westerns of total brain lysates.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.

Calidad

Routinely evaluated by Western Blot on Mouse brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.

Descripción de destino

50 kDa

Ligadura / enlace

Replaces: 04-1031; 04-1062

Forma física

Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Format: Purified
Protein A purified

Almacenamiento y estabilidad

Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
DO NOT FREEZE.

Nota de análisis

Control
Mouse brain tissue, Astrocyte culture.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Alemania

WGK 2

Certificado de Análisis

Certificado de origen

Alteration of glial-neuronal metabolic interactions in a mouse model of Alexander disease.
Meisingset, TW; Risa, ?; Brenner, M; Messing, A; Sonnewald, U
Glia null
Selective removal of photoreceptor cells in vivo using the biodegradable elastomer poly(glycerol sebacate).
Ghosh, F; Neeley, WL; Arner, K; Langer, R
Tissue Engineering: Part A null
Constitutive activation of Raf-1 induces glioma formation in mice.
Yelena Lyustikman, Hiroyuki Momota, William Pao, Eric C Holland
Neoplasia null
T Nishio et al.
Neuroscience, 132(1), 87-102 (2005-03-23)
Tenascin-C (TNC), an extracellular matrix glycoprotein, is involved in tissue morphogenesis like embryogenesis, wound healing or tumorigenesis. Astrocytes are known to play major roles in wound healing in the CNS. To elucidate the roles of TNC in wound closure by...
Pathogenic and nef-interrupted simian-human immunodeficiency viruses traffic to the macaque CNS and cause astrocytosis early after inoculation.
Singh, Dinesh K, et al.
Virology, 296, 39-51 (2002)
Artículos
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
Más información
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
Más información

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico