Merck
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MABF120

Sigma-Aldrich

Anti-EGFR Antibody, clone 225 (Azide-free)

clone 225, from mouse

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Sinónimos:
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

225, monoclonal

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable
neutralization: suitable

isotipo

IgG1κ

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

dry ice

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

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Este artículo
MABF119MABS829SAB4500721
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

225, monoclonal

clone

528, monoclonal

clone

3E7.2, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

rat, human, mouse

technique(s)

immunoprecipitation (IP): suitable, neutralization: suitable

technique(s)

immunoprecipitation (IP): suitable, neutralization: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000

isotype

IgG1κ

isotype

IgG2aκ

isotype

IgG2bκ

isotype

-

Descripción general

The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands including EGF, EPGN, TGFA, and EREG. Ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains which allow interaction with adaptor proteins and induction of several signaling pathways including PI3-AKT; PLCγ-PKC; Notch1; and Ras-Raf. EGFR therefore contributes to a diverse range of cellular processes inlcuding cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor.

Inmunógeno

Partially purified human EGFR from A431 cells

Aplicación

Anti-EGFR Antibody, clone 225 (Azide-free) is an antibody against EGFR for use in Immunoprecipitation, Neutralizing.
Neutralizing Assay Analysis: A representative lot was used by an independent laboratory in A431 cells. (Kawamoto, T., et al. (1984). Journal of Biological Chemistry. 259(12):7761-7766.)

Calidad

Evaluated by Immunoprecipitation in A431 cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated EGFR from A431 cell lysate.

Descripción de destino

~170 kDa observed. Uniprot gives a calculated molecular weight of 135 but can be observed at ~170 kDa due to phosphorylation. (Konishi, A., et al. (2003). The Journal of Biological Chemistry. 278(37):35049–35056.)

Ligadura / enlace

Replaces: 04-338

Forma física

Format: Purified

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable


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