MABS65

Sigma-Aldrich

Anti-Insulin Receptor Antibody, beta subunit, clone CT-3

clone CT-3, from mouse

Sinónimos:
CD220 antigen, insulin receptor
eCl@ss:
32160702
NACRES:
NA.41

Nivel de calidad

100

origen biológico

mouse

antibody product type

primary antibodies

clon

CT-3, monoclonal

species reactivity

rat (demonstrated by independent lab), human, mouse

aplicaciones

immunohistochemistry: suitable
western blot: suitable

isotipo

IgG1κ

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice

Descripción general

The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.

Especificidad

This antibody recognizes the C-terminus of human Insulin Receptor, beta subunit.

Inmunógeno

Recombinant protein corresponding to the C-terminus of human Insulin Receptor, beta subunit.
Epitope: C-terminus

Aplicación

Research Category
Metabolism
Detect Insulin Receptor using this Anti-Insulin Receptor Antibody, beta subunit, clone CT-3 validated for use in WB, IH.
Research Sub Category
Insulin/Energy Signaling

Calidad

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Insulin receptor beta subunit on 10 µg of mouse brain tissue lysate.

Descripción de destino

95 kDa

Forma física

Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Mouse brain tissue lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis
Rosaria Ottanà et al.
Molecules (Basel, Switzerland), 26(2) (2021-01-14)
Diabetes mellitus (DM) is a complex disease which currently affects more than 460 million people and is one of the leading cause of death worldwide. Its development implies numerous metabolic dysfunctions and the onset of hyperglycaemia-induced chronic complications. Multiple ligands...
Latha Devi et al.
PloS one, 7(3), e32792-e32792 (2012-03-10)
Although evidence is accumulating that diabetes mellitus is an important risk factor for sporadic Alzheimer's disease (AD), the mechanisms by which defects in insulin signaling may lead to the acceleration of AD progression remain unclear. In this study, we applied...
Yi Sui et al.
Stem cell research & therapy, 11(1), 86-86 (2020-02-28)
Parthenogenetic stem cells (PSCs) are a promising source of regenerated cardiomyocytes; however, their application may be limited without a paternal genome. Insulin-like growth factor-II (IGF-II), a paternally expressed growth hormone, is critical in embryonic differentiation. This study investigated whether forced...

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