OP03

Sigma-Aldrich

Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

liquid, clone PAb421, Calbiochem®

storage conditions

+2C to +8C

Nivel de calidad

100

origen biológico

mouse

antibody product type

primary antibodies

clon

PAb421, monoclonal

formulario

liquid

contiene

≤0.1% sodium azide as preservative

species reactivity

mouse, rabbit, monkey, rat, human

manufacturer/tradename

Calbiochem®

condiciones de almacenamiento

do not freeze

isotipo

IgG2a

enviado en

wet ice

Descripción general

Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.
This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins.

Inmunógeno

partially purified mouse p53
Mouse
Epitope: within amino acids 376-378 of human p53

Envase

20, 100 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

Aplicación

Frozen Sections (10 µg/ml, see application references)

Gel Shift (see comments)

Immunoblotting (10 µg/ml, see application references)

Immunofluorescence (1-20 µg/ml, see application references)

Flow Cytometry (1-20 µg/ml)

Immunoprecipitation (1 μg per sample, see application references)

Advertencia

Toxicity: Standard Handling (A)

Forma física

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Nota de análisis

Negative Control
SK-OV-3 cells or normal skin
Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)

Otras notas

Originally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Alemania

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis
Control of cell cycle progression by c-Jun is p53 dependent.
Schreiber, et al.
Genes & Development, 13, 607-619 (2019)
Mitomycin C and doxorubicin elicit conflicting signals by causing accumulation of cyclin E prior to p21WAF1/CIP1 elevation in human hepatocellular carcinoma cells.
Choi, et al.
International Journal of Oncology, 40, 277-286 (2013)
Identification and mapping of dimerization and DNA-binding domains in the C terminus of the IE2 regulatory protein of human cytomegalovirus.
Chiou, et al.
Journal of Virology, 67, 6201-6214 (2020)
Laura Fozzatti et al.
PloS one, 8(6), e67954-e67954 (2013-07-11)
Studies have suggested that the nuclear receptor corepressor 1 (NCOR1) could play an important role in human cancers. However, the detailed molecular mechanisms by which it functions in vivo to affect cancer progression are not clear. The present study elucidated...
Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response.
Bandhakavi, et al.
Molecular and Cellular Proteomics, 9, 403-414 (2021)

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