Merck
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PS1019

Sigma-Aldrich

PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb

liquid, Calbiochem®

Synonym(s):
Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr 183/185), Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr183/185)

origen biológico

rabbit

Nivel de calidad

100

antibody product type

primary antibodies

clon

polyclonal

formulario

liquid

contiene

≤0.1% sodium azide as preservative

species reactivity

human, rat, mouse

manufacturer/tradename

Calbiochem®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

isotipo

IgG

enviado en

wet ice

temp. de almacenamiento

−20°C

Gene Information

human ... MAPK8(5599)

Descripción general

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 proteins phosphorylated at Thr183 and Tyr185.
Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 phosphorylated at Thr183 and Tyr185 in uv-treated HEK293 cells.
This PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵).

Inmunógeno

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human JNK1 and JNK2

Envase

100 μL in Plastic ampoule

Aplicación


Immunoblotting (1:1000)

Advertencia

Toxicity: Standard Handling (A)

Forma física

In Dulbecco′s PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Nota de análisis

Positive Control
UV-treated HEK293 cells or sorbitol-treated PC12 cells

Otras notas

Choudhury, B.K., et al. 2002. J. Immunol.169, 5955.
Barr, R.K., et al. 2001. Int. J. Biochem. Cell. Biol.33, 1047.
Dong, C., et al. 2001. J. Clin. Immunol.21, 253.
Janulis, M., et al. 2001. Mol. Cell. Biol.21, 2235.
Pearce, A.K., et al. 2001. Trends Cell Biol.11, 426.
Tong, T., et al. 2001. Exp. Cell Res.269, 64.
Cobb, M.H., et al. 1999. Prog. Biophys. Mol. Biol.71, 479.
Basu, S., et al. 1998. Oncogene17, 3277.
The immunogen sequence is highly conserved among many species, including mouse, rat, chicken, nematode, and Drosophila, but cross-reactivity has not been determined. The immunogen sequence is also conserved in JNK3, but reactivity has not been detected. Antibody should be titrated for optimal results in individual systems.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Quality

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