S8005H

Sigma-Aldrich

CpGenome 5-hmC DNA Standard

CpGenome 5-hmC DNA St&ard contains a linear, double-str&ed DNA (897 bp) with 100% hydroxymethylated cytosine. This st&ard can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications.

Nivel de calidad

100

species reactivity

human

manufacturer/tradename

CpGenome
Upstate®

enviado en

dry ice

Categorías relacionadas

Descripción general

CpGenome 5-hmC DNA Standard is a linear, double-stranded DNA (897 bp) containing 100% 5-hydroxymethylcytosine. This standard can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications. This vial is a component of S8005 the CpGenome 5-mC and 5-hmC DNA Standard Set ( three linear, double-stranded DNAs (897 bp) with the same sequence, but containing either 100% 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines.

Materials Provided:

One vial containing 2 µg (40 µL) of CpGenome 5-hmC DNA Standard at a concentration of 50 ng/μL.

Validation:

Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.

CpGenome is a trademark of Serologicals Corporation.

Aplicación

Research Category
Epigenetics & Nuclear Function

Forma física

Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0

Almacenamiento y estabilidad

Recommended Storage: Stable for 6 months at -20°C from date of receipt.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

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