Merck
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10108014001

Roche

Papain

from Carica papaya

Synonym(s):
cystein protease, papain
Enzyme Commission number:

origen biológico

papaya

Nivel de calidad

100

formulario

suspension

specific activity

30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)

mol peso

23 kDa

envase

pkg of 10 mL (100 mg)

manufacturer/tradename

Roche

concentración

0.05-0.5 mg/mL

pH óptimo

6.0-7.0

enviado en

wet ice

Descripción general

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Aplicación

Papain has been used in the digestion of various brain cells.
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Acciones bioquímicas o fisiológicas

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Características y beneficios

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

Forma física

Suspension, crystalline, nonsterile

Nota de preparación

Working concentration: 0.05 to 0.5 mg/ml

Almacenamiento y estabilidad

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Otras notas

For life science research only. Not for use in diagnostic procedures.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

does not flash

Punto de inflamabilidad C

does not flash

Certificate of Analysis

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Certificate of Origin

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