Merck
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114391

Sigma-Aldrich

Bromophenol Blue

ACS reagent

Sinónimos:
3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form
Empirical Formula (Hill Notation):
C19H10Br4O5S
Número de CAS:
Peso molecular:
669.96
Beilstein:
61698
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.47

Nivel de calidad

200

grado

ACS reagent

formulario

powder

technique(s)

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

mp

273 °C (lit.)

solubilidad

ethanol: 0.1%

application(s)

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

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Categorías relacionadas

Descripción general

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Aplicación

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Envase

5, 25 g in glass bottle

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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