Merck
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51558

Sigma-Aldrich

HEPES buffered saline

BioUltra, for molecular biology, 2× concentrate

Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

Nivel de calidad

200

grado

for molecular biology

línea de producto

BioUltra

formulario

liquid

calidad

2× concentrate

composición

dextrose, 2.0 g/L
HEPES, 10 g/L
KCl, 0.74 g/L
NaCl, 16 g/L
Na2HPO4.2H2O, 0.27 g/L

concentración

2 ×

impurezas

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

índice de refracción

n20/D 1.339

pH

7.2 (25 °C)

trazas de catión

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

Absorción UV

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descripción general

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Aplicación

HEPES buffered saline has been used:
  • for the preparation of plasmid DNA
  • as a reagent to prepare transfection master mix for DNA construct vectors
  • to develop hydrogel precursor for cell leakage assay

Componentes

concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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