This product is intended for research use only and not suitable for GMP manufacturing. Currently, a GMP grade product option is not available.
I6758
IPTG
≥99% (TLC), ≤0.1% Dioxane
IPTG
Sinónimos:
Isopropyl β-D-1-thiogalactopyranoside, IPTG, Isopropyl β-D-thiogalactoside
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Fórmula empírica (notación de Hill):
C9H18O5S
Número CAS:
Peso molecular:
238.30
UNSPSC Code:
12352201
NACRES:
NA.31
PubChem Substance ID:
EC Number:
206-703-0
Beilstein/REAXYS Number:
4631
MDL number:
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BPHPUYQFMNQIOC-NXRLNHOXSA-N
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
SMILES string
CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
biological source
synthetic
description
non-ionic
assay
≥99% (TLC)
form
powder
technique(s)
thin layer chromatography (TLC): suitable
impurities
≤0.1% Dioxane
color
white to off-white
useful pH range
5-7 (50 g/L)
mp
105 °C ((221 °F))
solubility
water: 0.05 g/L, clear
application(s)
cell analysis
life science and biopharma
storage temp.
2-8°C
Quality Level
1 of 4
Este artículo | |||
|---|---|---|---|
| biological source synthetic | biological source - | biological source - | biological source - |
| assay ≥99% (TLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay - |
| technique(s) thin layer chromatography (TLC): suitable | technique(s) - | technique(s) - | technique(s) - |
| solubility water: 0.05 g/L, clear | solubility water: 50 mg/mL | solubility water: 100 mg/mL, methanol: soluble | solubility - |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| form powder | form solid | form solid | form liquid |
General description
Isopropyl β-D-Thiogalactopyranoside (IPTG) is a molecular biology reagent used to induce gene expression in bacterial systems. It mimics lactose, binding to the lac repressor protein, leading to gene activation. IPTG is crucial for studying gene regulation, protein expression, and recombinant protein production, providing valuable insights into cellular and molecular biology research.
Application
IPTG has been used:
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Biochem/physiol Actions
IPTG is a non-metabolizable analog of allolactose. It acts as an inducer that regulates the transcriptional activity of the lac promoter, a promoter that is used for the expression of the protein in E.coli.
Preparation Note
A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Non-metabolizable galactose analog.
To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B
Clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Listados normativos
Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.
123-91-1
CAS No.
123-91-1
CAS No.
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Artículos
General protocols for growth of competent cells and their transformation (uptake of DNA).
Protocolos
General protocols for growth of competent cells in microbial medium.
Contenido relacionado
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is this product suitable for GMP-batch use?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf¿Le ha resultado útil?
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How long and at what temperature is it stable after dissolving in water? Does it need to be stored at -20°C or 4°C?
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Following reconstitution, this material may be stored in aliquots at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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