P4864

Sigma-Aldrich

Propidium iodide solution

solution (1.0 mg/ml in water)

Sinónimos:
3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide
Empirical Formula (Hill Notation):
C27H34I2N4
Número de CAS:
Peso molecular:
668.39
Beilstein/REAXYS Number:
3843838
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.47

ensayo

≥94%

Nivel de calidad

200

formulario

solution (1.0 mg/ml in water)

aplicaciones

hematology: suitable
histology: suitable

solubilidad

water: 1 mg/mL

εmax

5400-6400 at 491-495 nm in phosphate buffer

Industria destacada

Diagnostic Assay Manufacturing

temp. de almacenamiento

2-8°C

SMILES string

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

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Descripción general

Propidium iodide solution is used to assess neuronal death.

Aplicación

Propidium iodide solution has been used:
  • in staining DNA
  • in the viability staining method to study islet viability
  • to visualize F-actins and nuclei in mesenchymal stem cell sheets

Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.

Envase

10 mL in glass bottle

storage_class_code

12 - Non Combustible Liquids

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves

Certificado de Análisis

Certificado de origen

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Propidium Iodide, Product P4864, stain viable or non-viable cells?

    Propidium Iodide does not generally enter living cells,  However it may be taken up by axonal terminals and retrogradely transported to neuronal cell bodies. Reference: Aschoff, A. and H. Hollander, Fluorescent compounds as retrograde tracers compared with horseradish peroxidase (HRP). I. A parametric study in the central visual system of the albino rat. J. Neurosci. Methods, 6(3), 179-197 (1982).

  4. What are the excitation and emission maxima for Propidium Iodide, Product P4864?

    Excitation maximum is at 493 nm in water.  Emission maximum is at 636 nm in water

  5. How is Propidium Iodide, Product P4864, used for Flow Cytometry?

    Immediately before flow cytometric analysis, prepare a 0.5 mg/mL solution of propidium iodide by combining 50μL of 2X PBS with 50 μL of P4864. Add this solution to 1 mL of a cell suspension containing 10,000,000 cells. (Note: Cells exhibiting fluorescence above 630 nm should be excluded from further analysis.)

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Iva Slaninová et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 71(9), 700-708 (2007-06-06)
Quaternary benzo[c]phenanthridine alkaloids (QBAs) are naturally occurring compounds isolated from plants in the Fumariaceae, Papaveraceae, Ranunculaceae, and Rutaceae families. In addition to having a wide range of biological activities, they are also attractive for their fluorescent properties. We observed interesting...
Stroke E-Book: Pathophysiology Diagnosis and Management, 79-79 (2011)
Yonggang Yang et al.
Scientific reports, 5, 18583-18583 (2015-12-22)
Viability is a common issue of concern in almost all microbial processes. Fluorescence-based assays are extensively used in microbial viability assessment, especially for mixed-species samples or biofilms. Propidium iodide (PI) is the most frequently used fluorescence indicator for cell viability...
Chung-Chi Wang et al.
Cardiovascular research, 77(3), 515-524 (2007-11-17)
Cell transplantation is a promising approach for patients with myocardial infarction. However, following injection, retention of the transplanted cells in the injected area remains a central issue, which can be deleterious to cell transplantation therapy. We hypothesized that the use...
Paternal cyclophosphamide exposure induces the formation of functional micronuclei during the first zygotic division
Grenier L, et al.
PLoS ONE, 6(11), e27600-e27600 (2011)
Artículos
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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