350R-1

Sigma-Aldrich

FoxP1 (SP133) Rabbit Monoclonal Antibody

NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

500
100

forma del anticuerpo

culture supernatant

antibody product type

primary antibodies

clon

SP133, monoclonal

descripción

For In Vitro Diagnostic Use in Select Regions (See Chart)

formulario

buffered aqueous solution

species reactivity

human

envase

vial of 0.1 mL concentrate (350R-14)
vial of 0.5 mL concentrate (350R-15)
bottle of 1.0 mL predilute (350R-17)
vial of 1.0 mL concentrate (350R-16)
bottle of 7.0 mL predilute (350R-18)

manufacturer/tradename

Cell Marque

aplicaciones

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500

isotipo

IgG

conjugado

unconjugated

control

DLBCL, lymph node, tonsil

enviado en

wet ice

temp. de almacenamiento

2-8°C

visualización

nuclear

Categorías relacionadas

Descripción general

Diffuse large B-cell lymphoma (DLBCL) represents different clinicopathologic entities which are difficult to separate using standard techniques. From the clinical standpoint, the introduction of immunochemotherapy in the treatment of DLBCL has dramatically improved the outcome of these patients compared with chemotherapy alone. Gene expression profiling (GEP) studies have shown that DLBCL can be reproducibly divided into the important subtypes of germinal center B-cell–like (GCB), activated B-cell–like (ABC), and unclassified DLBCL. It is beneficial to translate the GEP classification into protein expression by tumor cells through immunohistochemical (IHC) staining of formalin-fixed, paraffin-embedded tissues. A panel of antibodies: CD10, BCL6, MUM1/IRF4, GCET1, FoxP1, LMO2, and BCL2 has been used to determine GCB or ABC and each has different percentage thresholds for positive staining. Choi et al. demonstrated that the cases positive for GCET1 (≥ 80% of tumor cells) and MUM1/IRF4 (≥ 80%) and/or FoxP1 (≥ 80%) or negative for CD10 and BCL6 (≤ 30%) were assigned to the group. The cases positive for CD10 (≥ 30%), GCET1 (≥ 80%) without MUM1 expression, or positive for BCL6 without FoxP1 expression were classified as GCB. This study indicated the importance of FoxP1 in the subclassification of DLBCL. Choi et al then modified their approach to DLBCL subclassification by focusing on FoxP1. The tumors that are positive for both FoxP1 and GCET1 are assigned to GCB subgroup, but, if FoxP1 is positive and GCET1 is negative, the tumors belong to the ABC phenotype. If a case is FoxP1 negative but MUM-1/IRF4 positive, it still belongs to the ABC phenotype as long as CD10 is not expressed. This modified method emphasized the role of FoxP1, MUM1/IRF4, and GCET1 in the subclassification of DLBCL. The Choi′s algorithm had a very high concordance with the GEP results (87%). Therefore, FoxP1 is useful in subclassification of DLBCL and a high cutoff (≥80%) for FoxP1 is needed to achieve high specificity for the ABC subtype.

Calidad


IVD

IVD

IVD

RUO

Ligadura / enlace

FoxP1 Positive Control Slides, Product No. 350S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Forma física

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Otras notas

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Información legal

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis
Further studies on biosynthesis of erythropoietin.
R Bandyopadhyay et al.
Indian journal of biochemistry & biophysics, 18(4), 241-244 (1981-08-01)
Paul N Meyer et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(2), 200-207 (2010-12-08)
Patients with diffuse large B-cell lymphoma (DLBCL) can be divided into prognostic groups based on the cell of origin of the tumor as determined by microarray analysis. Various immunohistochemical algorithms have been developed to replicate these microarray results and/or stratify...
Michael Pfreundschuh et al.
The Lancet. Oncology, 7(5), 379-391 (2006-05-02)
The role of rituximab in combination with different CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone)-like chemotherapy regimens in young patients with good-prognosis diffuse large-B-cell lymphoma remains to be defined. We aimed to compare CHOP-like chemotherapy and rituximab with CHOP-like chemotherapy alone...
Thomas M Habermann et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 24(19), 3121-3127 (2006-06-07)
To address early and late treatment failures in older patients with diffuse large B-cell lymphoma (DLBCL), we designed a two-stage randomized trial of cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) versus rituximab plus CHOP (R-CHOP), with a second random assignment to...
Andreas Rosenwald et al.
The Journal of experimental medicine, 198(6), 851-862 (2003-09-17)
Using current diagnostic criteria, primary mediastinal B cell lymphoma (PMBL) cannot be distinguished from other types of diffuse large B cell lymphoma (DLBCL) reliably. We used gene expression profiling to develop a more precise molecular diagnosis of PMBL. PMBL patients...

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