47989

Sigma-Aldrich

Fura 2-AM

BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

Sinónimos:
Fura-2 pentakis(acetoxymethyl) ester
Empirical Formula (Hill Notation):
C44H47N3O24
Número de CAS:
Peso molecular:
1001.85
Beilstein/REAXYS Number:
8183748
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.32

Nivel de calidad

100

línea de producto

BioReagent

ensayo

≥95.0% (HPLC)

solubilidad

DMSO: soluble

fluorescencia

λex 355 nm; λem 495 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca2+

idoneidad

suitable for fluorescence

temp. de almacenamiento

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c4ncc(o4)C(=O)OCOC(C)=O

InChI

1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3

InChI key

VPSRLGDRGCKUTK-UHFFFAOYSA-N

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Aplicación

Fura-2 pentakis(acetoxymethyl) ester (Fura 2/AM), a fluorescence probe, is a membrane-permeable derivative of the calcium chelator Fura-2. Fura 2/AM is used to monitor intracellular levels of calcium under various physiological conditions.

Otras notas

Membrane-permeable derivative of Fura-2. Used to load the chelator into cells. Hydrolysed enzymatically in the cell to yield Fura-2 which remains within the cell

storage_class_code

13 - Non Combustible Solids

WGK Alemania

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

M Poenie et al.
Nature, 315(6015), 147-149 (1985-05-09)
Although the regulation of events in the cell division cycle by calcium or other cations has been the subject of much interest and speculation, experimental studies have been hampered by the difficulty of measuring submicromolar intracellular free calcium concentrations ([Ca2+]i)...
Robin R Hodges et al.
Experimental eye research, 103, 99-113 (2012-09-15)
The purpose of this study was to identify the signaling pathways that epidermal growth factor (EGF) uses to stimulate mucin secretion from cultured rat conjunctival goblet cells and to compare the pathways used by EGF with those used by the...
The use of microinjection and video microscopy for the study of calmodulin and calcium in living cells.
M Shelanski et al.
Methods in enzymology, 139, 824-834 (1987-01-01)
W Almers et al.
FEBS letters, 192(1), 13-18 (1985-11-11)
The Ca concentration ([Ca2+]i) in single rat peritoneal mast cells was measured by means of the new fluorescent Ca-indicator dye fura-2. Dye-loaded cells were made to degranulate with either antigen or compound 48/80. In cells loaded with extracellularly applied, membrane-permeant...
Jung Eun Lee et al.
European journal of immunology, 44(6), 1802-1813 (2014-03-13)
Along with MHC class I (MHCI), 2B4 provides nonredundant NK-cell inhibition in mice. The immunoregulatory role of 2B4 has been increasingly appreciated in models of tumor and viral infection, however, the interactions among 2B4, MHCI, and other activating NK-cell receptors...
Artículos
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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