49767

Glycerol

BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)

• Synonym(s): 1,2,3-Propanetriol, Glycerin
• Linear Formula: HOCH₂CH(OH)CH₂OH
• CAS Number: 56-81-5
• Molecular Weight: 92.09
• Beilstein: 635685
• Número de EC: 200-289-5
• Número MDL: MFCD00004722
• ID de la sustancia en PubChem: 57651220
• NACRES: NA.25

Properties

• grado: for molecular biology
• Nivel de calidad: 200
• densidad de vapor: 3.1 (vs air)
• presión de vapor: <1 mmHg ( 20 °C)
• Línea del producto: BioUltra
• ensayo: ≥99.5% (GC)
• formulario: viscous liquid
• temp. de autoignición: 698 °F
• impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• residuo de ign.: ≤0.1%
• índice de refracción: n20/D 1.474 (lit.); n20/D 1.474
• pH: 5.5-8 (25 °C, 5 M in H₂O)
• bp: 182 °C/20 mmHg (lit.)
• mp: 20 °C (lit.)
• solubilidad: H₂O: 5 M at 20 °C, clear, colorless
• densidad: 1.25 g/mL (lit.)
• trazas de anión: chloride (Cl^-): ≤1 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• trazas de catión: Ag: ≤5 mg/kg; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; NH₄⁺: ≤5 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: ≤1 mg/kg
• SMILES string: OCC(O)CO
• λ: 5 M in H₂O
• Absorción UV: λ: 260 nm A_max: 0.05; λ: 280 nm A_max: 0.07
• InChI: 1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
• InChI key: PEDCQBHIVMGVHV-UHFFFAOYSA-N

Description

Descripción general

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Aplicación

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Acciones bioquímicas o fisiológicas

Glycerol has been used as

• a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
• a fuel during designing enzymatic biofuel cell.
• a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
• a matrix for fast atom bombardment MS.
• may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Otras notas

Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal; Preparation of phage and plasmid DNA, for the storage of pure cultures

Safety Information

• Código de clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 1
• Punto de inflamabilidad F: 390.2 °F - Pensky-Martens closed cup
• Punto de inflamabilidad C: 199 °C - Pensky-Martens closed cup
• Equipo de protección personal: Eyeshields, Gloves