63689

2-Mercaptoethanol

BioUltra, for molecular biology, ≥99.0% (GC)

• Sinónimos: β-Mercaptoethanol, BME, Thioethylene glycol, 2-Hydroxyethylmercaptan
• Fórmula lineal: HSCH₂CH₂OH
• Número de CAS: 60-24-2
• Peso molecular: 78.13
• Beilstein: 773648
• Número de EC: 200-464-6
• Número MDL: MFCD00004890
• ID de la sustancia en PubChem: 57651953
• NACRES: NA.25

Propiedades

• Nivel de calidad: 200
• grado: for molecular biology
• densidad de vapor: 2.69 (vs air)
• presión de vapor: 1 mmHg ( 20 °C)
• línea de producto: BioUltra
• ensayo: ≥99.0% (GC)
• formulario: liquid
• lim. expl.: 18 %
• concentración: 14.3 M (pure liquid)
• technique(s): RNA extraction: suitable; protein extraction: suitable
• impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• índice de refracción: n20/D 1.500 (lit.); n20/D 1.501
• pH: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• solubilidad: H₂O: 0.5 M at 20 °C, clear, colorless
• densidad: 1.114 g/mL at 25 °C (lit.)
• trazas de catión: Al: ≤0.5 mg/kg; Ba: ≤0.1 mg/kg; Bi: ≤0.1 mg/kg; Ca: ≤0.5 mg/kg; Cd: ≤0.05 mg/kg; Co: ≤0.02 mg/kg; Cr: ≤0.02 mg/kg; Cu: ≤0.02 mg/kg; Fe: ≤0.1 mg/kg; K: ≤0.5 mg/kg; Li: ≤0.1 mg/kg; Mg: ≤0.1 mg/kg; Mn: ≤0.02 mg/kg; Mo: ≤0.1 mg/kg; Na: ≤1 mg/kg; Ni: ≤0.05 mg/kg; Pb: ≤0.1 mg/kg; Sr: ≤0.1 mg/kg; Zn: ≤0.1 mg/kg
• SMILES string: OCCS
• λ: 0.5 M in H₂O
• Absorción UV: λ: 260 nm A_max: 1.5; λ: 280 nm A_max: 0.3
• temp. de almacenamiento: 2-8°C
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Descripción

Aplicación

2-Mercaptoethanol has been used

• in the protein extraction buffer prepared for leaf samples.
• as a supplement in cell culture media.
• in the procedure of RNA extraction.

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Otras notas

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds

Información de seguridad

• pictogramas: GHS05,GHS06,GHS08,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410
• Consejos de prudencia: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Código de clase de almacenamiento: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Punto de inflamabilidad F: 165.2 °F - closed cup
• Punto de inflamabilidad C: 74 °C - closed cup
• Equipo de protección personal: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter