71736

Sodium dodecyl sulfate solution

BioUltra, for molecular biology, 10% in H₂O

• Sinónimos: Sodium lauryl sulfate solution, Lauryl sulfate sodium salt, SDS
• Fórmula lineal: CH₃(CH₂)₁₁OSO₃Na
• Número de CAS: 151-21-3
• Peso molecular: 288.38
• Beilstein: 3599286
• Número MDL: MFCD00036175
• eCl@ss: 39093306
• ID de la sustancia en PubChem: 57652355
• NACRES: NA.26

Propiedades

• grado: for molecular biology
• descripción: anionic
• línea de producto: BioUltra
• formulario: liquid
• mol peso: 288.38 g/mol
• concentración: 10% in H₂O
• technique(s): HPLC: suitable; protein quantification: suitable
• impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.0-6.5
• densidad: 1.01 g/mL at 20 °C
• trazas de anión: chloride (Cl^-): ≤50 mg/kg; phosphate (PO₄^3-): ≤1 mg/kg
• trazas de catión: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• Absorción UV: λ: 260 nm A_max: 0.3; λ: 280 nm A_max: 0.2
• SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Descripción

Aplicación

Sodium dodecyl sulfate solution has been used:

• in the preparation of 2x extraction buffer
• as a component in elution buffer for immunoprecipitation
• in SDS–polyacrylamide gel electrophoresis (PAGE) analysis

Acciones bioquímicas o fisiológicas

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations.

Otras notas

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material

Información de seguridad

• pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H315 - H319
• Consejos de prudencia: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Clasificaciones de peligro: Eye Irrit. 2 - Skin Irrit. 2
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 2
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter