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Quantitative RT-PCR ReadyMix™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

Sinónimos:

1-step RT-qPCR mix, Quantitative real-time PCR master mix

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Talla/SKUDisponibilidadPrecio
1 kit
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536,00 €

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NACRES:
NA.55
UNSPSC Code:
41106300

536,00 €


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Quality Segment

usage

sufficient for 250 reactions

feature

dNTPs included, hotstart

technique(s)

RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable

color

colorless

input

purified RNA

compatibility

ABI 5700, ABI 7000, ABI 7300, ABI 7500 Fast, ABI 7500, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7, Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ5, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex2 s, Eppendorf® Mastercycler ep realplex, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

General description

Quantitative RT-PCR ReadyMix™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Application

Quantitative RT-PCR ReadyMix™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).

Features and Benefits

  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced risk of contamination from multiple pipetting steps
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
  • Broad instrument compatibility
  • Includes a separate ROX reference dye vial for reaction normalization

Packaging

1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.

Legal Information

This product is for research use only.
Eppendorf is a registered trademark of Eppendorf SE
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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Este artículo
D7440L6669L6544
description

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

description

For probe-based real-time PCR

description

For fast probe-based quantitative PCR

description

For fast SYBR Green quantitative PCR

technique(s)

RT-qPCR: suitable, whole genome amplification: suitable

technique(s)

whole genome amplification: suitable, RT-qPCR: suitable

technique(s)

RT-qPCR: suitable, whole genome amplification: suitable

technique(s)

whole genome amplification: suitable, qPCR: suitable, RT-qPCR: suitable

color

colorless

color

colorless

color

colorless

color

colorless

feature

hotstart, dNTPs included

feature

hotstart, dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

usage

sufficient for 250 reactions

usage

sufficient for 400 reactions, sufficient for 20 reactions, sufficient for 100 reactions

usage

sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions

usage

sufficient for 2000 reactions, sufficient for 100 reactions, sufficient for 500 reactions

detection method

probe-based

detection method

probe-based

detection method

probe-based

detection method

SYBR™ Green

compatibility

Bio-Rad MyiQ, Bio-Rad MJ Opticon 2, ABI 7700, ABI 5700, Bio-Rad iQ5, ABI 7500, Illumina Eco qPCR, Cepheid SmartCycler, ABI StepOnePlus, Bio-Rad CFX96, Eppendorf® Mastercycler ep realplex2 s, Strategene Mx3000P, Bio-Rad MiniOpticon, Roche LightCycler 480, Bio-Rad MJ Chromo4, ABI ViiA 7, ABI 7900, ABI 7300, Strategene Mx4000, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene Q, Strategene Mx3005P, Qiagen Corbett Rotor-Gene 6000, Bio-Rad CFX384, Bio-Rad iCycler iQ, ABI 7000, ABI 7900 HT Fast, ABI 7500 Fast

compatibility

for use with ABI StepOnePlus, for use with Bio-Rad MJ Opticon, for use with ABI 7500 Fast, Bio-Rad MyiQ ( ), for use with Illumina Eco qPCR, for use with Bio-Rad MJ Chromo4, for use with Strategene Mx3000P, Bio-Rad iQ 5, for use with ABI 5700, for use with ABI 7900, for use with ABI 7900 Fast, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad CFX96, for use with Roche LightCycler 480, for use with Bio-Rad CFX384, for use with Bio-Rad MiniOpticon, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Strategene Mx4000, Bio-Rad iCycler iQ, for use with ABI 7900 HT, for use with ABI 7700, for use with Qiagen Corbett Rotor-Gene Q, for use with Strategene Mx3005P, for use with ABI 7000, for use with ABI ViiA 7, for use with Qiagen Corbett Rotor-Gene 3000, for use with ABI 7300, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Qiagen Corbett Rotor-Gene 6000

compatibility

for use with Bio-Rad MJ Opticon 2, for use with ABI 7900, for use with Bio-Rad CFX96, for use with Strategene Mx4000, for use with Roche LightCycler 480, for use with Qiagen Corbett Rotor-Gene Q, for use with ABI 7700, for use with ABI ViiA 7, for use with Strategene Mx3000P, for use with ABI 7500 Fast, for use with Eppendorf® Mastercycler ep realplex2 s, Bio-Rad iQ 5, for use with Bio-Rad CFX384, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with ABI 5700, for use with ABI 7000, for use with Qiagen Corbett Rotor-Gene 3000, Bio-Rad iCycler iQ, for use with Strategene Mx3005P, for use with ABI StepOnePlus, Bio-Rad MyiQ ( ), for use with ABI 7900 HT, for use with Qiagen Corbett Rotor-Gene 6000, for use with Bio-Rad MiniOpticon, for use with ABI 7300, for use with Illumina Eco qPCR, for use with ABI 7900 Fast

compatibility

for use with Bio-Rad MiniOpticon, for use with ABI StepOnePlus, for use with ABI ViiA 7, for use with Qiagen Corbett Rotor-Gene 6000, Bio-Rad iQ 5, for use with ABI 7500 Fast, for use with Bio-Rad CFX96, for use with Roche LightCycler 480, for use with Eppendorf® Mastercycler ep realplex, for use with ABI 5700, Bio-Rad iCycler iQ, for use with Qiagen Corbett Rotor-Gene 3000, for use with ABI 7300, for use with ABI 7900 HT, for use with Illumina Eco qPCR, for use with Strategene Mx3000P, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MJ Chromo4, for use with ABI 7000, Bio-Rad MyiQ, for use with Bio-Rad MJ Opticon 2, for use with Qiagen Corbett Rotor-Gene Q, for use with Strategene Mx4000, for use with ABI 7700, for use with Bio-Rad CFX384, for use with ABI 7900 Fast, for use with Strategene Mx3005P, for use with Eppendorf® Mastercycler ep realplex2 s


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Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1



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