A0729

Sigma-Aldrich

Monoclonal Anti-Activin B antibody produced in mouse

clone 146807, purified immunoglobulin

Número MDL:
NACRES:
NA.41

Nivel de calidad

100

origen biológico

mouse

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

146807, monoclonal

species reactivity

human

aplicaciones

neutralization: 0.6-3.0 μg/mL using Activin B-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. (ND50)
western blot: 1-2 μg/mL

isotipo

IgG1

conjugado

unconjugated

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Gene Information

human ... INHBB(3625)

Descripción general

Activins are pleotropic cytokines that belong to the TGF-β superfamily. There are three forms of activin, homodimeric activin A (βA-βA), activin B (βB-βB) and the heterodimeric activin AB (βA-βB). Activins bind to type I (ActRI) or type II (ActRII) receptor complexes. The cellular targets of Activin are Smad-2, -3 and -4 that form hetromeric complex and regulate transcription of target genes. The other pathways mediated by activins are Rho A, MAPK, p38 and JNK pathways. Activins play vital role in regulation of growth, differentiation, erythropoiesis, angiogenesis, survival or neural cells and tissue repair. Activin B is reported to promote wound healing by epithelial cell proliferation and migration via the RhoA-JNK signaling pathway. It is also vital in follicular development during the estrous cycle and normal fertility in female mammals. Various reports indicate the role of activin B in normal function of pancreatic β cells and in reducing the growth of renal cell carcinoma
Monoclonal Anti-Activin B recognizes human activin B.
Activins are members of the TGF-β family. Activins are dimeric proteins composed of 2 β subunits. Acitivn B contains 2 β B subunits. Activins bind to the Type II Activin receptor triggering phosphorylation of Type I Acitivn receptor (ALK4) and activation of downstream pathways such as proliferation and apoptosis.

Inmunógeno

purified, CHO cell-derived, recombinant human activin B.

Aplicación

Anti-Activin B antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The recommended working antibody concentration for ELISA is 0.5-1.0 μg/ml. For neutralization of human activin B expressed in K562 cell line, a concentration of 0.6-3.0 μg/ml is recommended.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Activin Signaling and Its Role in Regulation of Cell Proliferation, Apoptosis, and Carcinogenesis.
Chen, Y.
The Journal of Experimental Biology, 231, 534-544 (2006)
Sabine Werner et al.
Cytokine & growth factor reviews, 17(3), 157-171 (2006-02-17)
Activins are transforming growth factor beta superfamily members, which were originally identified as inducers of follicle-stimulating hormone release from the pituitary. In recent years, however, additional functions of activins have been discovered, including a role in the morphogenesis of various...
Yin Xia et al.
The Journal of endocrinology, 202(1), 1-12 (2009-03-11)
Activin was discovered in the 1980s as a gonadal protein that stimulated FSH release from pituitary gonadotropes and was thought of as a reproductive hormone. In the ensuing decades, many additional activities of activin were described and it was found...
Ingrid Wacker et al.
Molecular and cellular biology, 29(7), 1707-1718 (2009-01-23)
The von Hippel-Lindau tumor suppressor gene (VHL) is mutated in clear cell renal cell carcinomas (RCC), leading to the activation of hypoxia-inducible factor (HIF)-mediated gene transcription. Several VHL/HIF targets, such as glycolysis, angiogenesis, cell growth, and chemotaxis of tumor cells...
Philippe Bertolino et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(20), 7246-7251 (2008-05-16)
All major cell types in pancreatic islets express the transforming growth factor (TGF)-beta superfamily receptor ALK7, but the physiological function of this receptor has been unknown. Mutant mice lacking ALK7 showed normal pancreas organogenesis but developed an age-dependent syndrome involving...

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