Merck
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A1720

Sigma-Aldrich

G 418 disulfate salt

powder, BioReagent, suitable for cell culture

Synonym(s):
Antibiotic G418
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
CAS Number:
Molecular Weight:
692.71
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.76

línea de producto

BioReagent

Nivel de calidad

200

formulario

powder

actividad óptica

[α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))

potencia

≥720 μg per mg (Dried basis)

mol peso

692.7

envase

pkg of 1 g
pkg of 25 g
pkg of 5 g

technique(s)

cell culture | mammalian: suitable

color

white to off-white

solubilidad

H2O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)

espectro de actividad antibiótica

Gram-negative bacteria

Modo de acción

protein synthesis | interferes

temp. de almacenamiento

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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Descripción general

Chemical structure: aminoglycoside
G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.

Aplicación

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Envase

1, 5, 25 g in glass bottle

Acciones bioquímicas o fisiológicas

G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.

Precaución

The product is stable for two years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.

Nota de preparación

G418 powder is soluble in water at 100 mg/mL.

Otras notas

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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