A1949

Sigma-Aldrich

Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal

clone RG-96, purified from hybridoma cell culture

Sinónimos:
Monoclonal Anti-Rabbit IgG (γ-chain specific)
NACRES:
NA.46

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

purified immunoglobulin

antibody product type

secondary antibodies

clon

RG-96, monoclonal

formulario

lyophilized powder

species reactivity

rabbit

should not react with

chicken, guinea pig, goat, horse, sheep, rat, bovine, human, pig, feline, canine

envase

vial of 0.5 mL

aplicaciones

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)

isotipo

IgG1

conjugado

peroxidase conjugate

temp. de almacenamiento

2-8°C

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Descripción general

The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.
Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.

Inmunógeno

Rabbit IgG

Aplicación

Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in
  • various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
  • westernblotting
  • immunohistology
  • dot immunobinding assay

Forma física

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Nota de preparación

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

11 - Combustible Solids

WGK Alemania

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

NsrR: a key regulator circumventing Salmonella enterica serovar Typhimurium oxidative and nitrosative stress in vitro and in IFN-gamma-stimulated J774. 2 macrophages
Gilberthorpe NJ, et al.
Microbiology, 153(Pt 6), 1756-1756 (2007)
Targeted inhibition of EG-1 blocks breast tumor growth
Lu M, et al.
Cancer Biology & Therapy, 6(6), 936-941 (2007)
The rapid increase of circulating adiponectin in neonatal calves depends on colostrum intake
Kesser J, et al.
Journal of Dairy Science, 98(10), 7044-7051 (2015)
Daniel M Held et al.
Clinical and vaccine immunology : CVI, 17(6), 930-936 (2010-04-23)
New Zealand White (NZW) rabbits were immunized with several different nontoxic botulinum neurotoxin serotype B (BoNT/B) preparations in an effort to optimize the production of a rapid and highly potent, effective neutralizing antibody response. The immunogens included a recombinant heavy...
A W Horne et al.
Molecular human reproduction, 15(5), 287-294 (2009-03-14)
Ectopic pregnancy (EP) remains a considerable cause of morbidity and occasional mortality. Currently, there is no reliable test to differentiate ectopic from intrauterine gestation. We have previously used array technology to demonstrate that differences in gene expression in decidualized endometrium...

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