A2556

Sigma-Aldrich

Monoclonal Anti-Rabbit IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in mouse

clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution

Sinónimos:
Monoclonal Anti-Rabbit IgG (γ-chain specific)
NACRES:
NA.46

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

purified immunoglobulin

antibody product type

secondary antibodies

clon

RG-96, monoclonal

formulario

buffered aqueous glycerol solution

species reactivity

rabbit

should not react with

chicken, guinea pig, goat, horse, sheep, rat, bovine, human, pig, feline, canine

aplicaciones

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells

isotipo

IgG1

conjugado

alkaline phosphatase conjugate

enviado en

wet ice

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

Aplicación

Monoclonal Anti-Rabbit IgG (γ-chain specific)Alkaline Phosphatase antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Acciones bioquímicas o fisiológicas

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Forma física

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Alemania

WGK 2

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificado de Análisis

Certificado de origen

The Laboratory Rat (1998)
Regulation of the megakaryocytic glycoprotein IX promoter by the oncogenic Ets transcription factor Fli-1
Bastian LS, et al.
Blood, 93(8), 2637-2644 (1999)
The Immunoglobulins: Structure and Function (1998)
Molecular Genetics of Immunoglobulin, 17 (1987)
Serena Varotto et al.
Plant physiology, 133(2), 606-617 (2003-09-16)
We analyzed the expression profile and cellular localization of the maize (Zea mays) Rpd3-type histone deacetylases genes ZmRpd3/101, ZmRpd3/102, and ZmRpd3/108 (indicated as ZmHDA101, ZmHDA102, and ZmHDA108 in the Plant Chromatin Database). This study shows that maize Rpd3 transcripts are...

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