A4721

Sigma-Aldrich

Monoclonal Anti-ARNO (Cytohesin-2) antibody produced in mouse

~2 mg/mL, clone CYT2-21, purified immunoglobulin, buffered aqueous solution

Número MDL:
NACRES:
NA.41

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

CYT2-21, monoclonal

formulario

buffered aqueous solution

mol peso

antigen 40 kDa

species reactivity

rat, human

concentración

~2 mg/mL

aplicaciones

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 5-10 μg/mL using a whole extract of rat brain

isotipo

IgG1

conjugado

unconjugated

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Gene Information

human ... CYTH2(9266)
rat ... Cyth2(116692)

Descripción general

ARNO (Cytohesin-2) is a guanine-nucleotide exchange factor that belongs to Arf family of GTP-associated proteins. ARNO has been shown to modulate the movement of mouse preadipocyte 3T3-L1 cells. Studies have reported that cytohesin-2 is overexpressed in pulmonary cancers and can also serve as biomarker of prognosis in hepatocellular carcinomas.
Monoclonal Anti-ARNO (Cytohesin-2) reacts specifically with ARNO, and does not cross-react with other members of the cytohesin family. The antibody associates with human and rat ARNO.

Inmunógeno

recombinant, human ARNO (ARFnucleotide-binding-site opener).

Aplicación

To determine if ARNO binds to phosphoinositides, a protein-overlay assay was performed using monoclonal mouse anti-ARNO as the primary antibody for ARNO detection on membranes spotted with phosphoinositides and incubated with ARNO.
Mouse monoclonal anti-ARNO (Cytohesin-2) antibody can be used for western blot (5-10μg/mL using a whole extract of rat brain), microarray, indirect ELISA and immunocytochemistry applications.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania

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Kedong Xu et al.
Oncology reports, 29(6), 2211-2218 (2013-04-03)
Cytohesin-2 is overexpressed in human lung cancer and it activates cytoplasmic ErbB receptors. Inhibition of cytohesin-2 by SecinH3 reduces growth of EGFR-dependent lung cancer xenografts and improves the treatment of primarily EGFR-TKI-resistant lung cancers. Cytohesin-2 promotes HepG2 proliferation through the...
Tomohiro Torii et al.
The Journal of biological chemistry, 285(31), 24270-24281 (2010-06-08)
The formation of primitive adipose tissue is the initial process in adipose tissue development followed by the migration of preadipocytes into adipocyte clusters. Comparatively little is known about the molecular mechanism controlling preadipocyte migration. Here, we show that cytohesin-2, the...

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