Merck
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A8062

Sigma-Aldrich

Anti-Goat/Sheep IgG−Alkaline Phosphatase antibody, Mouse monoclonal

clone GT-34, purified from hybridoma cell culture

Sinónimos:
Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Alkaline Phosphatase antibody produced in mouse

origen biológico

mouse

conjugado

alkaline phosphatase conjugate

forma del anticuerpo

purified from hybridoma cell culture

antibody product type

secondary antibodies

clon

GT-34, monoclonal

formulario

buffered aqueous glycerol solution

species reactivity

bovine, sheep, goat

should not react with

rat, chicken, mouse, horse, feline, pig, canine, guinea pig, rabbit, human

technique(s)

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:150,000 using total cell extract of chicken fibroblasts (10-20 μg per well)

isotipo

IgG1

enviado en

wet ice

temp. de almacenamiento

2-8°C

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Descripción general

IgGs are glycoprotein antibodies that modulate several immune responses. Primary goat against target proteins are often used in various research applications. Thus, secondary anti-goat IgGs can be useful tools for the analysis of target proteins. However, secondary antibodies may lack species specificity as they may bind to non-related Igs in the test preparation. This may lead to increased levels of background staining. Hence, use of monoclonals can effectively reduce background staining and increase the specificity of immunoassays.
Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase recognizes goat and sheep IgGs. No cross-reactivity is observed with human IgGs or IgGs derived from the following species: guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, and cat.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

Inmunógeno

Purified goat IgG

Aplicación

The level of IL-5 in HPAEC conditioned medium was determined by ELISA using alkaline phosphatase-conjugated monoclonal mouse anti-goat/sheep IgG (GT-34) as the secondary antibody.
Mouse monoclonal anti-goat/sheep IgG-alkaline phosphatase antibody has been used as a secondary antibody for ELISA assays at 1:4,000 and 1:15,210 dilutions. The product can also be used for IHC (1:40) and western blot applications (1:150,000).

Forma física

Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificado de origen

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Quotes and Ordering

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Nina Brenden et al.
Journal of pharmaceutical sciences, 102(3), 1116-1124 (2013-01-15)
Avoiding unwanted immunogenicity is of key importance in the development of therapeutic drug proteins. Animal models are of less predictive value because most of the drug proteins are recognized as foreign proteins. However, different methods have been developed to obtain

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